无细胞体系中碱性磷酸酶合成的动力学及调控
Kinetics and regulation of cell-free alkaline phosphatase synthesis.
作者信息
Pratt C
出版信息
J Bacteriol. 1980 Sep;143(3):1265-74. doi: 10.1128/jb.143.3.1265-1274.1980.
Abstract
Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli has been observed. Synthesis from transducing phage deoxyribonucleic acid templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30) fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of beta-galactosidase. Alkaline phosphatase activity took longer to appear than beta-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.
摘要
已观察到在来自大肠杆菌的无细胞系统中碱性磷酸酶（EC 3.1.3.1）合成的调控情况。携带phoA⁺的转导噬菌体脱氧核糖核酸模板在野生型或碱性磷酸酶组成型突变体的S30组分中发生合成。在碱性磷酸酶阴性突变体（phoB）的S30组分中未发生合成。杂合基因phoA-lacZ也受phoB调控，这意味着phoA转录受到调节。无细胞合成中每个phoA⁺基因拷贝的活性碱性磷酸酶产量与β-半乳糖苷酶相似。碱性磷酸酶活性出现的时间比β-半乳糖苷酶活性长。碱性磷酸酶亚基的合成没有延迟，这表明在活性碱性磷酸酶形成之前会合成最少数量的亚基。
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