核RNA合成后不久就会添加多聚腺苷酸（poly(A)）。
Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.
作者信息
Salditt-Georgieff M, Harpold M, Sawicki S, Nevins J, Darnell J E
出版信息
J Cell Biol. 1980 Sep;86(3):844-8. doi: 10.1083/jcb.86.3.844.
Abstract
A kinetic analysis of the appearance of [3H]uridine label in RNA sequences that neighbor poly(A), as well as the incorporation of [3H]adenosine label into both the RNA chain and the poly(A) of poly(A)-containing molecules, shows that poly(A) is added within a minute or so after RNA chain synthesis in Chinese hamster ovary cells and HeLa cells. Previous conclusions by several groups (5-7) that poly(A) might be added as long as 20-30 min after RNA synthesis appear to be in error, and the present conclusion seems much more in line with several different types of recent studies with specific mRNAs that suggest prompt poly(A) addition (13-16).
摘要
对与多聚腺苷酸（poly(A)）相邻的RNA序列中[3H]尿苷标记出现情况的动力学分析，以及对含poly(A)分子的RNA链和poly(A)中[3H]腺苷标记掺入情况的分析表明，在中国仓鼠卵巢细胞和HeLa细胞中，poly(A)在RNA链合成后大约一分钟内添加。几个研究小组之前得出的结论（5 - 7），即poly(A)可能在RNA合成后长达20 - 30分钟才添加，似乎是错误的，而目前的结论似乎更符合最近针对特定mRNA的几种不同类型研究，这些研究表明poly(A)会迅速添加（13 - 16）。
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