早期腺病毒信使核糖核酸形成过程中多聚腺苷酸添加和剪接事件的顺序
Order of polyadenylic acid addition and splicing events in early adenovirus mRNA formation.
作者信息
Weber J, Blanchard J M, Ginsberg H, Darnell J E
出版信息
J Virol. 1980 Jan;33(1):286-91. doi: 10.1128/JVI.33.1.286-291.1980.
Abstract
A study of the processing of mRNA from two early adenovirus type 2 transcription units (regions 2 and 4 of adenovirus type 2 genome; [J. Flint, Cell 10:153--166, 1977]) revealed that polyadenylic acid [poly(A)] was added in most if not all cases to an unspliced nuclear RNA molecule whose coordinates extended from the apparent initiation site for RNA synthesis to the single poly(A) site in each transcription unit. An intermediate RNA molecule in the processing of the mRNA for the 72,000-M, single-stranded DNA binding protein showed that the first of the two intervening sequences, the one closest to the 5' end of the molecule, was removed first at least in the majority of the processed molecules. Finally, in cells labeled for 10 min and then treated with actinomycin D to stop further RNA synthesis, the majority, if not all, of the poly(A)-terminated nuclear RNA specific for region 2 was successfully processed and transported to the cytoplasm.
摘要
一项关于来自两个早期腺病毒2型转录单位（腺病毒2型基因组的2区和4区；[J. 弗林特，《细胞》10:153 - 166，1977]）的mRNA加工过程的研究表明，在大多数（即便不是所有）情况下，聚腺苷酸[poly(A)]会添加到一个未剪接的核RNA分子上，该分子的坐标从RNA合成的明显起始位点延伸至每个转录单位中的单个poly(A)位点。在加工72,000-M的单链DNA结合蛋白的mRNA过程中的一个中间RNA分子表明，两个间隔序列中的第一个，即最靠近分子5'端的那个，至少在大多数加工后的分子中是首先被去除的。最后，在用放线菌素D处理以停止进一步RNA合成之前标记10分钟的细胞中，大多数（即便不是所有）特定于2区的poly(A)末端核RNA都成功地进行了加工并转运到了细胞质中。
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Order of polyadenylic acid addition and splicing events in early adenovirus mRNA formation.早期腺病毒信使核糖核酸形成过程中多聚腺苷酸添加和剪接事件的顺序
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本文引用的文献
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Hydrolysis of polyadenylic acid by pancreatic ribonuclease.胰腺核糖核酸酶对聚腺苷酸的水解作用。
J Biol Chem. 1960 Aug;235:2393-8.
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Competition hybridization by "pre-saturation" of HeLa cell DNA.通过HeLa细胞DNA的“预饱和”进行竞争杂交。
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Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.在腺病毒复制过程中，向病毒特异性RNA添加聚腺苷酸序列。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
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Presence of cell and virus specific sequences in the same molecules of nuclear RNA from virus transformed cells.病毒转化细胞的核RNA同一分子中存在细胞和病毒特异性序列。
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Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines.腺病毒2型RNA序列在裂解感染细胞和转化细胞系中的定位
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:457-74. doi: 10.1101/sqb.1974.039.01.058.
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Short-lived messenger RNA in HeLa cells and its impace on the kinetics of accumulation of cytoplasmic polyadenylate.HeLa细胞中的短寿命信使核糖核酸及其对细胞质聚腺苷酸积累动力学的影响。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):389-93. doi: 10.1073/pnas.72.1.389.
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Capping of eucaryotic mRNAs.真核生物mRNA的加帽
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Nuclear transcripts larger than the cytoplasmic mRNAs are specified by segments of the adenovirus genome coding for early functions.大于细胞质信使核糖核酸的核转录本由腺病毒基因组中编码早期功能的片段所确定。
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10
The initiation sites for RNA transcription in Ad2 DNA.腺病毒2型（Ad2）DNA中RNA转录的起始位点。
Cell. 1977 Nov;12(3):733-9. doi: 10.1016/0092-8674(77)90273-2.
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