Stashenko P, Nadler L M, Hardy R, Schlossman S F
J Immunol. 1980 Oct;125(4):1678-85.
A human B lymphocyte-specific antigen (B1) was identified and characterized by the use of a monoclonal antibody. By indirect immunofluorescence, cytotoxicity, and quantitative absorption, B1 was present on approximately 9% of the peripheral blood mononuclear cell fraction and >95% of B cells from blood and lymphoid organs in all individuals tested. Monocytes, resting and activated T cells, null cells, and tumors of T cell and myeloid origin were B1 negative. B1 was distinct from standard B cell phenotypic markers, including Ig and Ia antigen. Removal of the B1 positive population in peripheral blood eliminated all B cells capable or responding to pokeweed mitogen by maturation to Ig-producing cells.
利用单克隆抗体鉴定并表征了一种人类B淋巴细胞特异性抗原（B1）。通过间接免疫荧光、细胞毒性和定量吸收法检测发现，在所有受试个体中，B1存在于约9%的外周血单个核细胞组分以及>95%的血液和淋巴器官中的B细胞上。单核细胞、静息和活化的T细胞、裸细胞以及T细胞和髓系来源的肿瘤细胞均为B1阴性。B1与包括Ig和Ia抗原在内的标准B细胞表型标志物不同。去除外周血中B1阳性群体可消除所有能够通过成熟为产生Ig的细胞而对商陆有丝分裂原产生反应的B细胞。
