Gilmartin M E, Culbertson V B, Freedberg I M
J Invest Dermatol. 1980 Sep;75(3):211-6. doi: 10.1111/1523-1747.ep12522887.
When human and rat epidermis are exposed to 32P-orthophosphoric acid, labeled phosphate is incorporated into several proteins. The pattern of phosphorylation is identical whether the isotope is delivered in vivo or in vitro. The predominant phosphorylated proteins are insoluble in Tris-HCl buffer but soluble in SDS-beta-mercaptoethanol. They migrate in SDS-polyacrylamide gels with apparent molecular weights between 45,000 and 65,000. When analyzed by two-dimensional gel electrophoresis, the labeled phosphoproteins co-migrate with keratins isolated from human callus. Serine is the phosphate acceptor in these proteins. The pattern of phosphorylation of these SDS-beta-mercaptoethanol soluble epidermal proteins is not changed in basal cell carcinoma, icthyosis vulgaris, Kyrle's disease or Netherton's syndrome. The pattern is altered in psoriasis. Others have demonstrated that a 63,000 molecular weight protein is absent from active psoriatic lesions. We have found that a 63,000 molecular weight phosphoprotein is present in uninvolved skin but absent in the psoriatic plaques.
当人和大鼠的表皮暴露于32P-正磷酸时，标记的磷酸盐会掺入几种蛋白质中。无论同位素是在体内还是体外递送，磷酸化模式都是相同的。主要的磷酸化蛋白不溶于Tris-HCl缓冲液，但可溶于SDS-巯基乙醇。它们在SDS-聚丙烯酰胺凝胶中迁移，表观分子量在45,000至65,000之间。通过二维凝胶电泳分析时，标记的磷蛋白与从人愈伤组织中分离的角蛋白共同迁移。丝氨酸是这些蛋白质中的磷酸受体。在基底细胞癌、寻常型鱼鳞病、凯尔氏病或 Netherton 综合征中，这些SDS-巯基乙醇可溶性表皮蛋白的磷酸化模式没有改变。在银屑病中该模式会发生改变。其他人已经证明，活跃的银屑病皮损中不存在分子量为63,000的蛋白质。我们发现，分子量为63,000的磷蛋白存在于未受累皮肤中，但在银屑病斑块中不存在。
