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[长时间运动活动并伴有睡眠剥夺的大鼠脊髓前角细胞中的核酸和蛋白质浓度]

[Nucleic acid and protein concentration in the anterior horn cells of the spinal cord in rats during prolonged motor activity coupled with sleep deprivation].

作者信息

Malikov U M

出版信息

Tsitologiia. 1980 Jun;22(6):666-9.

PMID:6158155
Abstract

The rats were placed in a cilindrical tretbahn 50 cm in diameter, being rotated with a constant speed of 2.3 RPM. After 24 and 48 hours of moving activity, the rats were decapitated, lumbal spinal cord being dissected, fixed and utilized for the cytospectrophotometrical determination of RNA and protein contents in neurone and glial cells. RNA content per a cell decreased by 24 and 12 per cent in neurones and glial cells, correspondingly, after the 24 hour moving activity. This content increased in neurones by 49 per cent and became normalized in glial cells after the 48 hour period. The protein content increased after the moving period by 57 and 20 per cent for neurones and glial cells, correspondingly.

摘要

将大鼠置于直径50厘米的圆柱形运动轨道中,以2.3转/分钟的恒定速度旋转。在进行24小时和48小时的运动活动后,将大鼠断头,解剖、固定腰段脊髓,并用于细胞分光光度法测定神经元和神经胶质细胞中的RNA和蛋白质含量。在进行24小时运动活动后,神经元和神经胶质细胞中每个细胞的RNA含量分别下降了24%和12%。在48小时后,神经元中的这种含量增加了49%,神经胶质细胞中的含量恢复正常。运动期后,神经元和神经胶质细胞中的蛋白质含量分别增加了57%和20%。

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