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[大鼠太空飞行后脊髓运动神经元和椎间神经节神经元中蛋白质和RNA的细胞化学研究]

[Cytochemical study of proteins and RNA in individual spinal cord motor neurons and intervertebral ganglion neurons of rats following space flight].

作者信息

Gorbunova A V, Portugalov V V

出版信息

Kosm Biol Aviakosm Med. 1977 Jul-Aug;11(4):24-8.

PMID:904281
Abstract

By the methods of quantitative cytochemistry the content of RNA cytoplasmatic protein was measured in motoneurons of anterior horns of the spinal cord and neurons of spinal ganglia of rats after their 22-day space flight. The exposure did not alter the content of RNA in motoneurons of the anterior horns of the spinal cord and reduced the value in large neurons of spinal ganglia. The flight diminished significantly the protein content in the cytoplasm of motoneurons and neurons of spinal ganglia as compared with the preflight level. On the 27th postflight day the RNA content in motoneurons of the anterior horns of the spinal cord increased and that in neurons of spinal ganglia did not differ from the preflight level. On the 27th postflight day the protein content in the cytoplasm of motoneurons returned to the preflight level and remained decreased in the cytoplasms of large neurons of spinal ganglia.

摘要

采用定量细胞化学方法,测定了大鼠在进行22天太空飞行后脊髓前角运动神经元和脊髓神经节神经元中RNA细胞质蛋白的含量。此次飞行并未改变脊髓前角运动神经元中RNA的含量,但降低了脊髓神经节中大神经元的RNA含量。与飞行前水平相比,太空飞行显著降低了运动神经元和脊髓神经节神经元细胞质中的蛋白质含量。在飞行后第27天,脊髓前角运动神经元中的RNA含量增加,而脊髓神经节神经元中的RNA含量与飞行前水平无差异。在飞行后第27天,运动神经元细胞质中的蛋白质含量恢复到飞行前水平,而脊髓神经节中大神经元细胞质中的蛋白质含量仍低于飞行前水平。

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