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莫桑比克罗非鱼(彼得斯)的垂体细胞学研究

Pituitary cytology of the teleost fish Tilapia mossambica (Peters).

作者信息

Joy K P, Sathyanesan A G

出版信息

Z Mikrosk Anat Forsch. 1980;94(2):337-44.

PMID:6158191
Abstract

In the pituitary of Tilapia mossambica, eight cell types were identified on the basis of their staining reactions. the RPD consists of erythrosinophils and PbH positive cells. PbH cells border the NH and give amphipilic reaction to tri- and tetrachrome dyes and Halmi's stain. Erythrosinophils are also stained with acid fuchsin and orange G. The PPD is made up of acidophils and cyanophils. The acidophils are stained with orange G, acid fuchsin and erythrosin. These cells form a palisade zone between the neurohypophysis and the cyanophils. The cyanophils are AB, AF, ATh, PAS and aniline blue positive. They include both thyrotrophs and gonadotrophs and cannot be differentiated. The gonadotrophs may be those which are degranulated and vacuolated in the breeding season. The PI is formed of PAS- and PbH positive cells which lack any definite pattern of arrangement. Apart from the chromophils, scattered chromophobes were seen throughout the adenohypophysis. Occasionally, cells resembling the cyanophils of PPD were noticed in the RPD and PI also.

摘要

在莫桑比克罗非鱼的脑垂体中,根据染色反应鉴定出了八种细胞类型。RPD由嗜酸性粒细胞和PbH阳性细胞组成。PbH细胞与NH相邻,对三色和四色染料以及哈尔米氏染色呈现双嗜性反应。嗜酸性粒细胞也被酸性品红和橙G染色。PPD由嗜酸性细胞和嗜碱性细胞组成。嗜酸性细胞被橙G、酸性品红和伊红染色。这些细胞在神经垂体和嗜碱性细胞之间形成一个栅栏状区域。嗜碱性细胞对AB、AF、ATh、PAS和苯胺蓝呈阳性反应。它们包括促甲状腺细胞和促性腺细胞,无法区分。促性腺细胞可能是在繁殖季节脱颗粒和空泡化的那些细胞。PI由PAS和PbH阳性细胞组成,这些细胞缺乏任何确定的排列模式。除了嗜色细胞外,在整个腺垂体中还可见散在的嫌色细胞。偶尔,在RPD和PI中也会注意到类似于PPD嗜碱性细胞的细胞。

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