Ruusuvaara P
Acta Ophthalmol (Copenh). 1980 Jun;58(3):440-53. doi: 10.1111/j.1755-3768.1980.tb05745.x.
This paper evaluates the numbers of corneal endothelial cells that survive transplantation. The donor endothelium was photographed with a specular microscope both before enucleation and in situ after keratoplasty. The cell density was measured in 16 corneas from donors with choroidal melanoma. Of these donor corneas 12 were cryopreserved, two were preserved in McCarey and Kaufman's (M-K) medium and two were transplanted fresh. The average follow-up period after keratoplasty was 11 months. The mean endothelial cell loss for the whole series was found to be 49%. The mean cell loss for the cryopreserved corneas was 55%. In the four other recipients, with donor corneas that had been stored in M-K medium or transplanted fresh, the mean cell loss was 32%. The corneas preserved in M-K medium had the highest cell density in the transplants, with cell losses of 21 and 22%. Cell losses in the two corneas transplanted fresh were 40 and 44%. Cell losses, in the cryopreserved grafts had a wide variation, 33-77%. No correlation could be found between cell loss and either the age of the donor or the duration of perservation. Freezing and thawing was found to damage a proportion of the cells so that they did not stain with para-nitro-blue tetrazolium (p-NBT) after preservation. Transmission and scanning electron microscopy also revealed changes in the intercellular spaces and some cell disruption in cryopreserved grafts.
本文评估了移植后存活的角膜内皮细胞数量。在摘除眼球前以及角膜移植术后原位，用镜面显微镜对供体角膜内皮进行拍照。对16只来自患有脉络膜黑色素瘤供体的角膜测量细胞密度。这些供体角膜中，12只进行了冷冻保存，2只保存在麦凯里和考夫曼（M-K）培养基中，2只新鲜移植。角膜移植术后的平均随访期为11个月。整个系列的平均内皮细胞损失率为49%。冷冻保存角膜的平均细胞损失率为55%。在其他4名接受者中，供体角膜保存在M-K培养基中或新鲜移植，平均细胞损失率为32%。保存在M-K培养基中的角膜在移植后细胞密度最高，细胞损失率分别为21%和22%。两只新鲜移植角膜的细胞损失率分别为40%和44%。冷冻保存移植物的细胞损失率差异很大，为33%-77%。未发现细胞损失与供体年龄或保存时间之间存在相关性。发现冷冻和解冻会损伤一部分细胞，使其在保存后不能用对硝基蓝四氮唑（p-NBT）染色。透射电子显微镜和扫描电子显微镜也显示冷冻保存移植物的细胞间隙有变化，且有一些细胞破坏。
