Ruusuvaara P
Acta Ophthalmol (Copenh). 1979 Oct;57(5):868-81. doi: 10.1111/j.1755-3768.1979.tb01854.x.
The endothelia of 102 clear human corneal grafts in 95 patients were photographed in vivo with a specular microscope. The endothelial cell densities of the grafts were counted from a area of 0.01 mm2. The follow-up period averaged three years. The patients' ages ranged from 16 to 77 years and that of the donors from 8 to 89 years. The average endothelial cell density for all 102 transplants was 1169 +/- 478 cells/mm2. Corneal transplants stored in MK medium (N = 19) have more cells than cryopreserved grafts (N = 42) or grafts stored in the moist chamber (N = 41); this last group was used as control material. The differences in cell densities were statistically significant. The cell density of cryopreserved grafts was significantly higher than in grafts stored in moist chamber. Only in those grafts in which moist chamber storage had been used was endothelial cell density dependent on time of storage. With an increase in cadaver time (time from death to enucleation), the endothelial cell density of the graft decreased. An inverse correlation was found between the cell density of the graft and donor age. Good results were still found with healthy donors over 60 years. The endothelial cell density of the grafts showed a steady decrease during the long-term post-operative period, the rate of cell loss being much higher in graft endothelium than in normal endothelium during ageing.
用镜面显微镜对95例患者的102片透明人角膜移植片的内皮进行了活体拍照。从0.01mm²的区域计数移植片的内皮细胞密度。随访期平均为三年。患者年龄在16至77岁之间，供体年龄在8至89岁之间。所有102片移植片的平均内皮细胞密度为1169±478个细胞/mm²。保存在MK培养基中的角膜移植片（N = 19）比冷冻保存的移植片（N = 42）或保存在湿盒中的移植片（N = 41）有更多的细胞；最后一组用作对照材料。细胞密度的差异具有统计学意义。冷冻保存的移植片的细胞密度显著高于保存在湿盒中的移植片。只有在使用湿盒保存的那些移植片中，内皮细胞密度才取决于保存时间。随着尸体时间（从死亡到摘除眼球的时间）的增加，移植片的内皮细胞密度降低。发现移植片的细胞密度与供体年龄呈负相关。60岁以上的健康供体仍能取得良好的效果。在术后长期，移植片的内皮细胞密度呈稳定下降，在老化过程中，移植片内皮的细胞丢失率远高于正常内皮。
