Nakagawa T, Blaser K, de Weck A L
Int Arch Allergy Appl Immunol. 1980;63(2):212-9. doi: 10.1159/000232629.
The effect of hapten density on benzylpenicilloyl (BPO)-protein conjugates upon the induction of BPO-specific IgE immune response was investigated in BALB/c and C3H mice. Bovine gamma-globulin (BGG) and ovalbumin (OA) were employed as carrier proteins. In both strains of mice, moderately substituted conjugates (BPO19-BGG, and BPO-OA with an epitope density from 2 to 7.5) elicited a persistent BPO-specific IgE, while heavily substituted conjugates (BPO38-BGG) induced only a transient response. In contrast, lightly substituted conjugates (BPO3.2-, BPO9.5-BGG, and BPO1-OA) failed to produce BPO-specific IgE antibodies. There was no significant difference in carrier-specific IgE immune response among these various conjugates. These results suggest that moderately substituted hapten-protein conjugates are one of the favorable conditions for eliciting hapten-specific IgE immune response in mice.
在BALB/c和C3H小鼠中研究了半抗原密度对苄基青霉素酰（BPO）-蛋白质偶联物诱导BPO特异性IgE免疫反应的影响。使用牛γ球蛋白（BGG）和卵清蛋白（OA）作为载体蛋白。在这两种品系的小鼠中，中等取代的偶联物（BPO19-BGG以及表位密度为2至7.5的BPO-OA）引发了持续的BPO特异性IgE，而高度取代的偶联物（BPO38-BGG）仅诱导了短暂的反应。相比之下，轻度取代的偶联物（BPO3.2-、BPO9.5-BGG和BPO1-OA）未能产生BPO特异性IgE抗体。这些不同偶联物之间在载体特异性IgE免疫反应方面没有显著差异。这些结果表明，中等取代的半抗原-蛋白质偶联物是在小鼠中引发半抗原特异性IgE免疫反应的有利条件之一。
