Steenbergen C, Williamson J R
Adv Myocardiol. 1980;2:271-84.
The distribution of coronary flow during high-flow hypoxia and ischemia has been assessed in the perfused rat heart. Surface and cross-section NADH fluorescence photography defined the pattern of oxygen delivery because regions of high NADH fluorescence denote areas of anoxia. Thioflavin S, a fluorescent dye that stains vascular endothelium, was added to the perfusate to label perfused vessels. The results indicate 1) that relatively large anoxic zones develop during high-flow hypoxia or ischemia, 2) that the pattern of oxygen delivery under hypoxic conditions is reproducible, and 3) that flow into these anaerobic areas is significantly decreased relative to control. The border zone between aerobic and anoxic tissue is shown to be sharply defined, indicating the absence of an appreciable volume of tissue that is partially anaerobic. The data may be relevant to human coronary artery disease, where coronary occlusion is usuallly not complete. They suggest that with reduced, but not insignificant, oxygen delivery into the domain of a coronary artery, the distribution of flow would be heterogeneous, making small areas of tissue severely ischemic while preserving flow and oxygen supply in the adjacent tissue.
在灌注的大鼠心脏中评估了高流量低氧和缺血期间冠状动脉血流的分布情况。表面和横截面的NADH荧光摄影确定了氧气输送模式，因为高NADH荧光区域表示缺氧区域。将硫黄素S（一种对血管内皮进行染色的荧光染料）添加到灌注液中以标记灌注血管。结果表明：1）在高流量低氧或缺血期间会形成相对较大的缺氧区域；2）低氧条件下的氧气输送模式具有可重复性；3）相对于对照组，流入这些无氧区域的血流量显著减少。有氧和无氧组织之间的边界区域显示出清晰的界定，表明不存在明显体积的部分无氧组织。这些数据可能与人类冠状动脉疾病相关，在人类冠状动脉疾病中冠状动脉阻塞通常并不完全。它们表明，当进入冠状动脉区域的氧气输送减少但并非微不足道时，血流分布将是不均匀的，使得小面积组织严重缺血，而相邻组织的血流和氧气供应得以保留。
