用甲基绿-派洛宁对尿道分泌物中的淋病奈瑟菌进行一步染色。
One-step staining of Neisseria gonorrhoeae in urethral discharge by methyl green-pyronin.
作者信息
Hanna N F, Taylor-Robinson D, Csonka G W, Harris J R, Al-Sowaygh I A
出版信息
Br J Vener Dis. 1980 Aug;56(4):227-9. doi: 10.1136/sti.56.4.227.
Methyl green-pyronin (MGP) was used in a one-step procedure to stain smears of urethral discharge from 169 men. Duplicate smears were stained by Gram's method and discharge was cultured for Neisseria gonorrhoeae. The organisms were isolated from 67 specimens and intracellular diplococci were seen in 74 smears after Gram staining and in 77 after staining by MGP. Furthermore, more extracellular and intracellular diplococci were seen in smears stained by MGP than by Gram's method and the proportion of polymorphonuclear leucocytes found to contain the organisms was greater after staining with MGP. Staining with MGP is simple, rapid, inexpensive, and easily automated.
采用甲基绿-派洛宁（MGP）一步法对169名男性尿道分泌物涂片进行染色。同时，将涂片进行重复革兰氏染色，并对尿道分泌物进行淋病奈瑟菌培养。从67份标本中分离出了该菌，革兰氏染色后在74份涂片及MGP染色后在77份涂片上可见细胞内双球菌。此外，MGP染色涂片上可见的细胞外和细胞内双球菌比革兰氏染色涂片上更多，且MGP染色后发现含有该菌的多形核白细胞比例更高。MGP染色操作简单、快速、成本低且易于自动化。
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