Hashimoto T, Suzuku S, Hasegawa T, Sakakibara N, Endo M, Kobayashi S, Takemoto T
Endoscopy. 1980 Sep;12(5):205-10. doi: 10.1055/s-2007-1021744.
To elucidate the developmental process of the intestinal metaplasia of the stomach, PNNG was orally administered to dogs and the sequential endoscopic examination was performed by using the methylene blue staining method. In the early period of the drug administration dogs showed the findings of erosive gastritis at the antral portion. In the 42nd experimental week, a clearly stained lesion was observed at the gastric angle. The histological findings of the biopsy specimens from the stained lesion showed the elongation of the foveolar epithelium and goblet cell-like mucous cells. Apparently stained lesion was observed at the antral portion in the 128th week. The close-up endoscopic appearance was very similar to that of stained intestinal metaplasia seen in the human stomach. The endoscopic methylene blue staining method as performed in the experimental dogs could provide useful results in the observation of minute lesions especially intestinal metaplasia in human stomach. This experiment, and further studies, may help to elucidate the pathogenesis of the gastric carcinoma in humans.
为阐明胃肠化生的发育过程，给犬口服N-甲基-N'-硝基-N-亚硝基胍（PNNG），并采用亚甲蓝染色法进行连续内镜检查。给药初期，犬胃窦部出现糜烂性胃炎的表现。在实验第42周，胃角处观察到明显染色的病变。来自染色病变的活检标本的组织学结果显示胃小凹上皮伸长和杯状细胞样黏液细胞。在第128周时，胃窦部观察到明显染色的病变。内镜特写外观与在人胃中看到的染色肠化生非常相似。在实验犬中进行的内镜亚甲蓝染色法在观察微小病变尤其是人胃中的肠化生方面可提供有用的结果。该实验及进一步的研究可能有助于阐明人类胃癌的发病机制。
