Strauss D, de Verdier C D
Folia Haematol Int Mag Klin Morphol Blutforsch. 1980;107(3):434-53.
Purine nucleotides of fresh human red cells and of red cells during storage at 4 degrees and 25 degrees C with additions of adenine, guanine, guanosine and inosine were estimated by HPLC. Six nucleotides were found in red cells: ATP, ADP, AMP, GTP, GDP, and IMP. The adenine nucleotides represented 92 per cent of the total purine nucleotides, guanine nucleotides 7 per cent and IMP less than 1 per cent. In red cells stored with adenine the total concentration of purine nucleotides increased to 125 per cent of the normal value. An adenine-free but guanine and guanine + inosine containing medium caused a decrease of the concentration of purine nucleotides by 10 to 20 per cent. When red cells were stored without adding guanine or guanosine the content of the guanine nucleotides decreased from 0.32 to 0.17 mumol/g Hb due to the decrease in the GTP content, but the GDP concentration increased slightly. In CPD-AG blood, however, the concentration of guanine nucleotides increased considerably up to 0.6 mumol/g Hb. IMP was estimated in all investigated stored red cells. In CPD-A and in CPD-AG blood 0.4 mumol/g Hb were produced during 3 weeks of storage, but twice of that in CPD-AI blood. The principles of the synthesis and the degradation of purine nucleotides in stored red cells are discussed in detail.
采用高效液相色谱法对新鲜人红细胞以及在4℃和25℃下添加腺嘌呤、鸟嘌呤、鸟苷和肌苷储存期间的红细胞中的嘌呤核苷酸进行了测定。在红细胞中发现了六种核苷酸：ATP、ADP、AMP、GTP、GDP和IMP。腺嘌呤核苷酸占总嘌呤核苷酸的92%，鸟嘌呤核苷酸占7%，IMP不到1%。在添加腺嘌呤储存的红细胞中，嘌呤核苷酸的总浓度增加到正常值的125%。不含腺嘌呤但含有鸟嘌呤和鸟嘌呤+肌苷的培养基会使嘌呤核苷酸浓度降低10%至20%。当红细胞在不添加鸟嘌呤或鸟苷的情况下储存时，由于GTP含量降低，鸟嘌呤核苷酸含量从0.32μmol/g Hb降至0.17μmol/g Hb，但GDP浓度略有增加。然而，在CPD-AG血液中，鸟嘌呤核苷酸浓度大幅增加至0.6μmol/g Hb。对所有研究的储存红细胞中的IMP进行了测定。在CPD-A和CPD-AG血液中，储存3周期间产生了0.4μmol/g Hb，但在CPD-AI血液中产生的量是其两倍。详细讨论了储存红细胞中嘌呤核苷酸合成和降解的原理。
