Fouad F M, Waldron-Edward D
Hoppe Seylers Z Physiol Chem. 1980 May;361(5):703-13. doi: 10.1515/bchm2.1980.361.1.703.
High molecular weight glycoproteins were isolated and purified from canine antral and fundic mucosal tissue by means of non-degrading techniques. The results disclosed the advantage of urea extraction technique over the culture method in isolating the native glycoproteins. The glycoproteins were susceptible to degradation by protease, thus yielding low molecular weight glycopeptides. Chemical analysis of these glycopeptides and their parent macromolecules revealed that the oligosaccharide residues are attached to threonine, serine and proline residues of the protein chains. Similarly, high molecular weight glycoproteins isolated from human gastric gel mucin showed the same characteristics of canine gastric glycoproteins. Canine fundic glycoprotein or glycopeptide released their prosthetic carbohydrate groups under the lytic effect of fundic acid hydrolases.
通过非降解技术从犬胃窦和胃底黏膜组织中分离并纯化出高分子量糖蛋白。结果表明，在分离天然糖蛋白方面，尿素提取技术优于培养法。这些糖蛋白易被蛋白酶降解，从而产生低分子量糖肽。对这些糖肽及其母体大分子的化学分析表明，寡糖残基与蛋白质链中的苏氨酸、丝氨酸和脯氨酸残基相连。同样，从人胃凝胶粘蛋白中分离出的高分子量糖蛋白也具有犬胃糖蛋白的相同特征。犬胃底糖蛋白或糖肽在胃底酸水解酶的裂解作用下释放出其辅基碳水化合物基团。
