Sidransky H, Verney E, Murty C N
J Nutr. 1980 Nov;110(11):2231-42. doi: 10.1093/jn/110.11.2231.
The effect of the administration of L-tryptophan or tryptophan-related compounds on rat liver RNA and protein metabolism was investigated. The five biochemical parameters studied were polyribosomal aggregation, protein synthesis in vitro, synthesis of cytoplasmic poly(A)-mRNA, release of labeled nuclear RNA in vitro (nuclear and cell sap effects) and nuclear envelope nucleoside triphosphatase activity. The administration of L-tryptophan to overnight fasted rats revealed a rapid stimulation in all of the five parameters. Also, some of the tryptophan-related compounds, especially 5-hydroxy-DL-tryptophan, 3-indolepyruvic acid, indole or 3-hydroxyanthranilic acid, revealed stimulation in many of the parameters. However, when rats were pretreated with puromycin to inhibit protein synthesis, only the administration of L-tryptophan was still able to stimulate significantly the hepatic polyribosomes, in vitro protein synthesis, in vitro nuclear RNA release (involving nuclei but not cell sap) and nuclear envelope nucleoside triphosphatase activity. The effects by the other tryptophan-related compounds became inhibited. The results emphasize the unique action of L-tryptophan on hepatic protein synthesis.
研究了给予L-色氨酸或色氨酸相关化合物对大鼠肝脏RNA和蛋白质代谢的影响。所研究的五个生化参数分别为多核糖体聚集、体外蛋白质合成、细胞质聚腺苷酸（poly(A)）-mRNA的合成、体外标记核RNA的释放（核及细胞液效应）以及核膜核苷三磷酸酶活性。给禁食过夜的大鼠给予L-色氨酸后，所有这五个参数均迅速受到刺激。此外，一些色氨酸相关化合物，尤其是5-羟基-DL-色氨酸、3-吲哚丙酮酸、吲哚或3-羟基邻氨基苯甲酸，在许多参数上也表现出刺激作用。然而，当用嘌呤霉素预处理大鼠以抑制蛋白质合成时，只有给予L-色氨酸仍能显著刺激肝脏多核糖体、体外蛋白质合成、体外核RNA释放（涉及细胞核而非细胞液）以及核膜核苷三磷酸酶活性。其他色氨酸相关化合物的作用则受到抑制。这些结果强调了L-色氨酸对肝脏蛋白质合成的独特作用。
