Mello M L
Histochem J. 1980 Sep;12(5):499-511. doi: 10.1007/BF01011924.
Feulgen absorption spectra of polytene chromosome regions, differing in types of DNA composition and of chromatin compactness, were determined microspectrophotometrically in squashed salivary glands of Rhynchosciara americana. The absorption curves exhibited a secondary maximum at their ascending branch after the chromatins were maximally depurinated. The degree of shoulder prominence could not be specifically correlated with the packing state or even with richness in satellite repetitive DNA of the chromatins. It is assumed that other factors may exist which play a part in the phenomenon that gives rise to the Feulgen absorption shoulder characteristics. Differences in the extraction rate of the apurinic acid fragments due to their binding to specific non-histone proteins are suggested as contributing to the above-mentioned absorption patterns when comparing the various chromatin types.
利用显微分光光度法，在美洲大鼻蝇的压碎唾液腺中测定了多线染色体区域的孚尔根吸收光谱，这些区域在DNA组成类型和染色质紧密程度上存在差异。在染色质最大程度脱嘌呤后，吸收曲线在其上升分支处出现了一个次最大值。肩部突出的程度与染色质的包装状态甚至卫星重复DNA的丰富程度都没有特定的相关性。据推测，可能存在其他因素导致了产生孚尔根吸收肩部特征的现象。当比较各种染色质类型时，由于脱嘌呤酸片段与特定非组蛋白的结合而导致的提取率差异，被认为是上述吸收模式的原因之一。
