连续自动测定从体外灌注大鼠唾液腺释放的α-淀粉酶

Continuous automated assay of alpha-amylase release from superfused rat salivary gland.

作者信息

Templeton D

出版信息

J Pharmacol Methods. 1980 Nov;4(3):189-95. doi: 10.1016/0160-5402(80)90010-8.

Abstract

A method of continuous automated amylase assay is described. This relies on the absorption of iodine by starch to produce a blue color that can be quantified colorimetrically. Digestion of the starch by amylase released from parotid tissue slices reduces the intensity of the color formed, allowing quantification of the amylase released. The assay in sensitive to 0.05 U/amylase/ml and linear up to 13 U/ml. Typical tissue responses to acetyl-beta-methylcholine and isoprenaline are presented.

摘要

本文描述了一种连续自动淀粉酶测定方法。该方法依赖于淀粉对碘的吸收以产生可通过比色法定量的蓝色。腮腺组织切片释放的淀粉酶对淀粉的消化会降低所形成颜色的强度，从而能够对释放的淀粉酶进行定量。该测定法对0.05 U/淀粉酶/毫升敏感，在高达13 U/毫升的范围内呈线性。文中展示了典型组织对乙酰-β-甲基胆碱和异丙肾上腺素的反应。