Thammapalerd N, Savanat T
Southeast Asian J Trop Med Public Health. 1980 Sep;11(3):378-83.
Two methods used for the purification of specific antigen for amoebiasis were affinity chromatography and agarose gel electrophoresis. The IgG fraction of the serum from a patient with amoebic liver abscess showing a specific band in the IEP test was coupled to CNBr-activated Sepharose 4B. After passage through the immunoadsorbent column of the crude or partially purified extract of Entamoeba histolytica, followed by acid dissociation, specific antigen was obtained. This antigen gave only one band in the IEP test against the serum which gave multiple bands upon testing with the crude antigen. With the agarose gel electrophoresis, fraction 2 from the slit was found to contain specific antigen. Rabbits immunized with the excised agarose strip of this fraction produced antibodies giving 2 precipitating bands in the IEP test, one of which was specific for amoebiasis.
用于纯化阿米巴病特异性抗原的两种方法是亲和层析和琼脂糖凝胶电泳。来自一名阿米巴肝脓肿患者的血清中，在免疫电泳试验中显示出特异性条带的IgG部分与溴化氰活化的琼脂糖4B偶联。通过溶组织内阿米巴粗提物或部分纯化提取物的免疫吸附柱后，经酸解离，获得了特异性抗原。该抗原在免疫电泳试验中与用粗抗原检测时出现多条条带的血清反应，仅产生一条条带。通过琼脂糖凝胶电泳，发现狭缝处的第2部分含有特异性抗原。用该部分切下的琼脂糖条带免疫的兔子产生的抗体在免疫电泳试验中产生2条沉淀带，其中一条对阿米巴病具有特异性。
