Pillai S, Mohimen A
Gastroenterology. 1982 Dec;83(6):1210-6.
A sensitive and specific immunochemical assay for Entamoeba histolytica antigens would be a valuable tool for clinical diagnosis, to study the sequelae of amoebiasis, and to screen for the expression of amoebic proteins in recombinant bacterial clones. The major impediment toward developing such an assay is the cross-reactivity of anti-E. histolytica antisera with a wide range of mammalian serum proteins. A rabbit anti-E. histolytica antiserum was repeatedly passed over three bovine serum protein immunoadsorbents and affinity purified over an E. histolytica protein-Sepharose 4B matrix. The purified antibody was radiolabeled and formed the upper layer of two specific and sensitive sandwich radioimmunoassays for amoebic proteins. One assay used a rabbit antiamoebic antibody solid phase and the other a human antibody solid phase. The latter assay proved capable of detecting amoebic antigens in the polyethylene glycol precipitates of sera from 21 of 21 patients with amoebiasis (and none of 22 control subjects).
一种针对溶组织内阿米巴抗原的灵敏且特异的免疫化学检测方法，对于临床诊断、研究阿米巴病的后遗症以及筛选重组细菌克隆中阿米巴蛋白的表达而言，将是一种有价值的工具。开发这样一种检测方法的主要障碍是抗溶组织内阿米巴抗血清与多种哺乳动物血清蛋白的交叉反应性。将兔抗溶组织内阿米巴抗血清反复通过三种牛血清蛋白免疫吸附剂，并在溶组织内阿米巴蛋白 - 琼脂糖4B基质上进行亲和纯化。纯化后的抗体进行放射性标记，并构成了两种针对阿米巴蛋白的特异且灵敏的夹心放射免疫测定法的上层。一种测定法使用兔抗阿米巴抗体固相，另一种使用人抗体固相。后一种测定法能够检测21例阿米巴病患者血清的聚乙二醇沉淀物中的阿米巴抗原（22例对照受试者均未检测到）。
