Barnett R I, Lyons G W, Driscoll J D, Forrest W J
Stain Technol. 1980 Jul;55(4):235-9. doi: 10.3109/10520298009067246.
Brain sectioning has been improved through gelatin embedding so that more than forty precisely oriented serial sections can be obtained from a single brain. Since the embedding gelatin requires no fixation, it can be removed from the sections prior to staining by simple warming. The reduction of Berlin blue dye commonly observed after staining by the Mulligan method has been found to be at least partly due to light in the UV to near UV range. Dye reduction is significantly inhibited by postfixation in 25% acetic acid.
通过明胶包埋改进了脑切片技术，这样从单个大脑中可以获得四十多个精确取向的连续切片。由于包埋明胶不需要固定，在染色前通过简单加热就可以从切片上去除。已经发现，用穆里根方法染色后常见的柏林蓝染料减少至少部分是由于紫外线到近紫外线范围内的光。在25%乙酸中进行后固定可显著抑制染料减少。
