[采用凝集试验法测定霍乱弧菌神经氨酸酶]
[Use of the aggregate hemagglutination method for determining cholera vibrio neuraminidase].
作者信息
Vertiev Iu V, Ezepchuk Iu V, Gorina L G
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1980 Nov(11):60-4.
The suitability of the aggregate hemagglutination test for neuraminidase determination in biological fluids has been evaluated. This test allows one to determine neuraminidase at a concentration of 2--4 mg/ml. The sensitivity of this test is more than 2 orders higher than that of gel diffusion technique and the thiobarbituric assay. The test has revealed that neuraminidase produced by Vibrio cholerae is not immunologically related to other kinds of neuraminidase produced by Corynebacterium diphtheriae, Clostridium perfringes and Erysepelothrix insidiosa.
已对用于生物体液中神经氨酸酶测定的凝集试验的适用性进行了评估。该试验可测定浓度为2-4毫克/毫升的神经氨酸酶。此试验的灵敏度比凝胶扩散技术和硫代巴比妥酸测定法高两个多数量级。该试验表明，霍乱弧菌产生的神经氨酸酶与白喉棒状杆菌、产气荚膜梭菌和败血丹毒丝菌产生的其他种类神经氨酸酶无免疫相关性。
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