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小鼠骨髓的形态学。

The morphology of the bone marrow of the mouse.

作者信息

de Cock P A, Tanke H J, Schröder-Van der Elst J P

出版信息

Acta Histochem. 1980;67(1):95-106. doi: 10.1016/S0065-1281(80)80059-6.

Abstract

For an investigation on the morphology of the cells of the bone marrow of the mouse, use was made of 2 stains: MAY-GRUNWALD-GIEMSA (MGG) and methyl-green-pyronine-stilbene (MPS). The results are reported, and are compared with each other and with those obtained with the LENDRUM stain. To compare results in the same cell, its coordinates were determined with a computer-controlled scanning microscope after staining with the first method, and the preparation was then destained and restained for evaluation of the second method. The advantages and disadvantages of the stains are discussed, and it is concluded that for the evaluation of the morphology of the eosinophilic leukocyte, the MPS stain seems to be superior.

摘要

为了研究小鼠骨髓细胞的形态,使用了两种染色剂:MAY-GRUNWALD-GIEMSA(MGG)和甲基绿-派洛宁-二苯乙烯(MPS)。报告了结果,并将其相互比较,还与用LENDRUM染色剂获得的结果进行了比较。为了比较同一细胞中的结果,在用第一种方法染色后,用计算机控制的扫描显微镜确定其坐标,然后将标本脱色并重新染色以评估第二种方法。讨论了这些染色剂的优缺点,得出的结论是,对于嗜酸性粒细胞形态的评估,MPS染色剂似乎更具优势。

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