Zheng Z X, Tang Y, Fang J, Peng X, Fan J D, Cui H M, Yang L Z
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Colleage of Biological and Agro-forestry Engineering, Tongren University, Tongren, Guizhou, 554300, China.
Anat Histol Embryol. 2017 Feb;46(1):17-24. doi: 10.1111/ahe.12225. Epub 2016 Jan 19.
The ultrastructural and cytochemical properties of peripheral blood cells of Gymnocypris eckloni were investigated by transmission electron microscopy and a range of cytochemical techniques to provide clear insight into the structure and function of blood cells from this fish. Ultrastructurally, erythrocytes, leucocytes (neutrophils, eosinophils, lymphocytes, monocytes), thrombocytes and plasma cells were identified in the peripheral blood of G. eckloni. The most special ultrastructural characteristics of blood cells in this fish were that neutrophils exhibited only one type of cytoplasmic granules containing an eccentric, spherical or oval electron-dense core, and eosinophils presented two types of granules with non-uniform electronic density and without crystalloids in their cytoplasm. Neutrophils, eosinophils, lymphocytes, monocytes and thrombocytes were positive for periodic acid-Schiff and α-naphthyl acetate esterase staining. Intense peroxidase positive staining was observed in neutrophils and monocytes, but not in eosinophils, lymphocytes and thrombocytes. Neutrophils, eosinophils and monocytes were stained positively for acid phosphatase, whereas lymphocytes and thrombocytes did not stain. Leucocytes and thrombocytes were negative for alkaline phosphatase and Sudan black B staining. Erythrocytes were negative for all cytochemical staining. The cytochemical and ultrastructural features of peripheral blood cells of G. eckloni were similar to those of other fish species. However, some important differences were identified in G. eckloni.
运用透射电子显微镜和一系列细胞化学技术，对青海湖裸鲤外周血细胞的超微结构和细胞化学特性进行了研究，以深入了解这种鱼类血细胞的结构和功能。在超微结构上，青海湖裸鲤外周血中可识别出红细胞、白细胞（中性粒细胞、嗜酸性粒细胞、淋巴细胞、单核细胞）、血小板和浆细胞。这种鱼血细胞最特殊的超微结构特征是，中性粒细胞仅表现出一种细胞质颗粒，含有一个偏心的、球形或椭圆形的电子致密核心，嗜酸性粒细胞呈现出两种电子密度不均匀且细胞质中无晶体的颗粒。中性粒细胞、嗜酸性粒细胞、淋巴细胞、单核细胞和血小板对过碘酸-希夫反应和α-萘乙酸酯酶染色呈阳性。在中性粒细胞和单核细胞中观察到强烈的过氧化物酶阳性染色，但在嗜酸性粒细胞、淋巴细胞和血小板中未观察到。中性粒细胞、嗜酸性粒细胞和单核细胞对酸性磷酸酶染色呈阳性，而淋巴细胞和血小板不着色。白细胞和血小板对碱性磷酸酶和苏丹黑B染色呈阴性。红细胞对所有细胞化学染色均呈阴性。青海湖裸鲤外周血细胞的细胞化学和超微结构特征与其他鱼类相似。然而，在青海湖裸鲤中也发现了一些重要差异。
