Berstad J
Acta Pharmacol Toxicol (Copenh). 1980 Sep;47(3):213-6. doi: 10.1111/j.1600-0773.1980.tb01562.x.
Dextran (9 mg/ml incubate) was found to induce a serotonin (5-HT) release from isolated rat peritoneal mast cells of about 10% within an incubation period of 5 min. at 37 degrees. The extent of release was not increased by using higher concentrations of dextran or by increasing the incubation period from 5 to 20 min. The dextran-induced release of 5-HT was increased to 50-60% when the mast cells were preincubated with phosphatidylserine (PS) in doses of 2.6-4.3 microgram/ml incubate. Higher concentrations of PS alone induced a rapid 5-HT release of approximately 50-60%. The 5-HT release process studied was found to possess both similarities and dissimilarities with the previously described dextran-induced release process of histamine, also studied in isolated rat peritoneal mast cells. The results supported previous indirect evidence of a 5-HT release from mast cells in the early phase of the dextran-induced anaphylactoid reaction in the rat.
发现右旋糖酐（9毫克/毫升孵育液）在37摄氏度下孵育5分钟内可诱导分离的大鼠腹膜肥大细胞释放约10%的血清素（5-羟色胺，5-HT）。使用更高浓度的右旋糖酐或将孵育时间从5分钟延长至20分钟，释放程度并未增加。当肥大细胞与2.6 - 4.3微克/毫升孵育液剂量的磷脂酰丝氨酸（PS）预孵育时，右旋糖酐诱导的5-HT释放增加到50 - 60%。单独使用更高浓度的PS可诱导约50 - 60%的快速5-HT释放。研究发现，所研究的5-HT释放过程与先前在分离的大鼠腹膜肥大细胞中研究的右旋糖酐诱导的组胺释放过程既有相似之处，也有不同之处。这些结果支持了先前关于大鼠右旋糖酐诱导的类过敏反应早期肥大细胞释放5-HT的间接证据。
