Makler M T, Pesce A J
Am J Clin Pathol. 1980 Nov;74(5):673-6. doi: 10.1093/ajcp/74.5.673.
An enzyme-linked immunosorbent assay (ELISA) for the measurement of nanogram quantities of hemoglobin A and hemoglobin F is described. The assay employs hemoglobin bound to polystyrene test tubes. The antigen is quantitated by the addition of rabbit antibody specific for the hemoglobin type, followed by a second antibody of goat antirabbit gamma globulin coupled to alkaline phosphatase. Quantitation of the reaction is achieved by incubating the coated tubes with p-nitrophenyl phosphate. The binding characteristics, the linearity, the ability of the assay to discriminate between hemoglobin A and hemoglobin F, the ability to discriminate between native and denatured gamma globin, and the comparison of this assay with a previously described immunoradiometric (IRMA) assay are discussed.
本文描述了一种用于测定纳克量血红蛋白A和血红蛋白F的酶联免疫吸附测定法（ELISA）。该测定法采用结合在聚苯乙烯试管上的血红蛋白。通过加入针对特定血红蛋白类型的兔抗体，然后加入与碱性磷酸酶偶联的山羊抗兔γ球蛋白二抗来定量抗原。通过将包被的试管与对硝基苯磷酸酯孵育来实现反应的定量。讨论了结合特性、线性、该测定法区分血红蛋白A和血红蛋白F的能力、区分天然和变性γ球蛋白的能力，以及该测定法与先前描述的免疫放射测定法（IRMA）的比较。
