Taxy J B, Mendelsohn G, Gupta P K
Am J Clin Pathol. 1980 Dec;74(6):791-5. doi: 10.1093/ajcp/74.6.791.
The argyrophil and argentaffin staining reactions of the intracytoplasmic granules of 23 carcinoid tumors of the rectum were studied. The granules were evaluated for the presence of amines by means of formalin-induced fluorescence and an immunoperoxidase method for serotonin. The results indicate that most rectal carcinoids are argyrophil when the more sensitive Grimelius method is employed. Only occasionally are rectal carcinoids argentaffin, a feature that correlates with positive fluorescent reactions and suggests amine content. Of three argentaffin tumors, one stained positively for serotonin, and a second contained clusters of pleomorphic electron-dense granules of the type more typically seen in known amine-containing carcinoid tumors. For practical purposes, the Grimelius argyrophil stain is most useful in confirming a light-microscopic diagnosis of a rectal carcinoid.
对23例直肠类癌肿瘤细胞质内颗粒的嗜银和亲银染色反应进行了研究。通过福尔马林诱导荧光和血清素免疫过氧化物酶法评估颗粒中胺的存在情况。结果表明，当采用更敏感的格里梅利乌斯方法时，大多数直肠类癌为嗜银性。直肠类癌仅偶尔为亲银性，这一特征与阳性荧光反应相关，并提示存在胺类物质。在三例亲银性肿瘤中，一例血清素染色呈阳性，另一例含有多形性电子致密颗粒簇，这种颗粒类型在已知含胺类的类癌肿瘤中更常见。实际上，格里梅利乌斯嗜银染色在直肠类癌的光镜诊断中最有用。
