一种通过高效液相色谱同时分析未修饰和修饰的尿核苷及核苷碱基的新方法。
A new method for the simultaneous analysis of unmodified and modified urinary nucleosides and nucleobases by high performance liquid chromatography.
作者信息
Schöch G, Thomale J, Lorenz H, Suberg H, Karsten U
出版信息
Clin Chim Acta. 1980 Dec 8;108(2):247-57. doi: 10.1016/0009-8981(80)90011-x.
PMID:6160930
Abstract
Details are given of a new, rapid and simple pre-fractionation method and an isocratic high performance liquid chromatography system suitable for parallel analysis of nucleosides and nucleobases from urine and other biological fluids. The quantitative recovery and excellent reproducibility of the method is demonstrated by analysis of representative standard RNA catabolites. The advantage of this new method for application to biological samples is discussed.
摘要
介绍了一种新型、快速且简单的预分离方法以及一种等度高效液相色谱系统，该系统适用于对尿液和其他生物体液中的核苷和核碱基进行平行分析。通过对代表性标准RNA分解代谢产物的分析，证明了该方法的定量回收率和出色的重现性。讨论了这种新方法应用于生物样品的优势。
相似文献
1
A new method for the simultaneous analysis of unmodified and modified urinary nucleosides and nucleobases by high performance liquid chromatography.一种通过高效液相色谱同时分析未修饰和修饰的尿核苷及核苷碱基的新方法。
Clin Chim Acta. 1980 Dec 8;108(2):247-57. doi: 10.1016/0009-8981(80)90011-x.
2
An improved method for the simultaneous analysis of normal and modified urinary nucleosides and nucleobases by high performance liquid chromatography.一种通过高效液相色谱同时分析正常和修饰的尿核苷及核苷碱基的改进方法。
Clin Chim Acta. 1985 Nov 15;152(3):355-61. doi: 10.1016/0009-8981(85)90111-1.
3
Hydrophilic interaction ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides, nucleosides and nucleobases in Ziziphus plants.亲水作用超高效液相色谱-串联三重四极杆质谱法测定枣属植物中的核苷酸、核苷和碱基。
J Chromatogr A. 2013 Aug 2;1301:147-55. doi: 10.1016/j.chroma.2013.05.074. Epub 2013 Jun 6.
4
Dynamic cation-exchange systems for rapid separations of nucleobases and nucleosides by high-performance liquid chromatography.
J Chromatogr. 1981 May 22;209(3):369-76. doi: 10.1016/s0021-9673(00)80604-4.
5
High-performance liquid chromatographic method for the analysis of purine and pyrimidine bases, ribonucleosides and deoxyribonucleosides in biological fluids.用于分析生物体液中嘌呤和嘧啶碱基、核糖核苷及脱氧核糖核苷的高效液相色谱法。
J Chromatogr. 1985 May 3;339(2):380-7. doi: 10.1016/s0378-4347(00)84667-8.
6
Quantitation of urinary nucleosides by high-performance liquid chromatography.高效液相色谱法定量测定尿核苷
J Chromatogr A. 1997 Feb 28;763(1-2):193-7. doi: 10.1016/s0021-9673(96)00757-1.
7
Improved and rapid method for quantitation of modified nucleosides in urine and sera with Radial-Pak cartridge.采用径向 Pak 柱定量尿液和血清中修饰核苷的改良快速方法。
J Chromatogr. 1986 Jan 10;374(1):149-54. doi: 10.1016/s0378-4347(00)83264-8.
8
Use of a fundamental elution protocol for the development of reversed-phase high-performance liquid chromatography enabling rapid simultaneous determination of purines, pyrimidines and allied compounds commonly found in human biological fluids.使用基本洗脱方案开发反相高效液相色谱法，可快速同时测定人生物体液中常见的嘌呤、嘧啶及相关化合物。
J Chromatogr. 1985 Nov 8;344:101-13. doi: 10.1016/s0378-4347(00)82011-3.
9
High-performance liquid chromatographic separation and quantitation of nucleosides in urine and some other biological fluids.
Clin Chem. 1977 Aug;23(8):1427-35.
10
Reversed-phase high-performance liquid chromatographic investigation of urinary normal and modified nucleosides of cancer patients.癌症患者尿液中正常和修饰核苷的反相高效液相色谱研究。
J Chromatogr B Biomed Sci Appl. 1999 Sep 24;732(2):307-13. doi: 10.1016/s0378-4347(99)00296-0.
引用本文的文献
1
Excretion of modified nucleosides during development of malignant lymphomas in mice after whole body irradiation.全身照射后小鼠恶性淋巴瘤发生发展过程中修饰核苷的排泄情况。
J Cancer Res Clin Oncol. 1984;108(3):302-7. doi: 10.1007/BF00390462.
Zotero 插件