Brachet J, de Petrocellis B, Ficq A, Alexandre H
Differentiation. 1980;17(2):63-76. doi: 10.1111/j.1432-0436.1980.tb01082.x.
Activation of unfertilized Chaetopterus eggs by treatment with an excess of KCl may lead to the production of unicellular ciliated larvae (Lillie's differentiation without cleavage). The effects of a number of inhibitors of protein (puromycin, cycloheximide, emetin), RNA (actinomycin D), and DNA (hydroxyurea) synthesis of differentiation without cleavage and on normal development have been studied in Chaetopterus. Incorporation of radioactive leucine, uridine, and thymine has been followed by biochemical methods and by autoradiography. The DNA content of the large polyploid nucleus has been estimated by cytophotometry. The initial pseudocleavage period of differentiation without cleavage is characterized by a burst in DNA and protein synthesis; the inhibitors have little or no effect on this burst and on pseudocleavage itself. Protein and DNA synthesis levels off during the following phase (segregation), but the inhibitors become more effective. RNA synthesis is almost linear for 20 h. These results are compared with those obtained on eggs from other species.
用过量氯化钾处理未受精的毛翼虫卵可使其激活，进而产生单细胞纤毛幼虫（利利氏无卵裂分化）。已在毛翼虫中研究了多种蛋白质（嘌呤霉素、放线菌酮、吐根碱）、RNA（放线菌素D）和DNA（羟基脲）合成抑制剂对无卵裂分化及正常发育的影响。通过生化方法和放射自显影追踪了放射性亮氨酸、尿苷和胸腺嘧啶的掺入情况。通过细胞光度法估算了大型多倍体细胞核的DNA含量。无卵裂分化的初始假卵裂期的特点是DNA和蛋白质合成激增；抑制剂对这种激增和假卵裂本身几乎没有影响。在随后的阶段（分离期），蛋白质和DNA合成趋于平稳，但抑制剂变得更有效。RNA合成在20小时内几乎呈线性。将这些结果与从其他物种的卵中获得的结果进行了比较。
