Segal J, Gordon A
Endocrinology. 1977 Jul;101(1):150-6. doi: 10.1210/endo-101-1-150.
The uptake of 2-deoxy-D-[1-3H]glucose ([1-3H]DOG) by cultured chick embryo heart cells was stimulated by L-triiodothyronine (T3). This stimulation began with the exposure to T3, rose linearly for about 8 h and reached a plateau around 24h. Actinomycin D, puromycin and cyclohexmide had no effect on the stimulated sugar uptake during the first 6h. These agents prevented the further increase in the rate of [1-3H]DOG uptake in T3-treated cultures that occurred between 6 and 24h. In the presence of hydroxyurea the uptake of [1-3H]DOG in both control and T3-treated cells was unchanged. These results suggest that some of the effects of T3 may not require new synthesis of either RNA, proteins or DNA.
L-三碘甲状腺原氨酸（T3）可刺激培养的鸡胚心脏细胞摄取2-脱氧-D-[1-³H]葡萄糖（[1-³H]DOG）。这种刺激从接触T3开始，在约8小时内呈线性上升，并在24小时左右达到平台期。放线菌素D、嘌呤霉素和环己酰亚胺在前6小时对刺激的糖摄取没有影响。这些药物可阻止在6至24小时之间T3处理的培养物中[1-³H]DOG摄取速率的进一步增加。在存在羟基脲的情况下，对照细胞和T3处理细胞中[1-³H]DOG的摄取均未改变。这些结果表明，T3的某些作用可能不需要新合成RNA、蛋白质或DNA。
