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使逆转录酶与多精胺核糖核酸酶形成复合物。

Complexing reverse transcriptase with polyspermine-ribonuclease.

作者信息

Bandyopadhyay A K, Wang D, Levy C C

出版信息

Biochem J. 1980 Jul 1;189(1):89-93. doi: 10.1042/bj1890089.

DOI:10.1042/bj1890089
PMID:6161606
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161920/
Abstract

Polyspermine-ribonuclease (Mr approximately 17 000) and the enzyme transcriptase from Rauscher-leukaemia virus (Mr approximately 70 000) form a complex Mr approx. 160 000) such that the molar ratio of polyspermine-ribonuclease to reverse transcriptase is 5:1. The most favourable condition for complex-formation is in a solution consisting of 0.01 M-Tris/HCl buffer, pH 7.5, 0.25 M-KCl and 1 mM-Mn2+ at 37 degrees C. The association of the two enzymes retains full RNAase activity, but reverse-transcriptase activity is completely inhibited when ribonuclease-sensitive polymers such as (dG)12 x (rC)n or viral 70S RNA are used as primer templates.

摘要

多精胺核糖核酸酶（分子量约为17000）与劳氏肉瘤病毒的酶转录酶（分子量约为70000）形成一种复合物（分子量约为160000），使得多精胺核糖核酸酶与逆转录酶的摩尔比为5:1。形成复合物的最适宜条件是在由0.01M Tris/HCl缓冲液（pH 7.5）、0.25M KCl和1mM Mn²⁺组成的溶液中，于37℃下进行。这两种酶的结合保留了全部的核糖核酸酶活性，但当使用对核糖核酸酶敏感的聚合物如（dG）12×（rC）n或病毒70S RNA作为引物模板时，逆转录酶活性被完全抑制。

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本文引用的文献

1
Complementary nuclear RNA's of murine sarcoma-leukemia virus complex in transformed cells.转化细胞中鼠肉瘤-白血病病毒复合体的互补核RNA
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1372-9. doi: 10.1073/pnas.64.4.1372.
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Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen.致癌核糖核酸病毒的结构蛋白。种间II，一种新的种间抗原。
J Biol Chem. 1973 Aug 25;248(16):5627-33.
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Comparison of antitumor activities of pancreatic ribonuclease and its cross-linked dimer.胰腺核糖核酸酶及其交联二聚体的抗肿瘤活性比较。
Cancer Res. 1976 Nov;36(11 Pt 1):4074-8.
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Preparation of cross-linked dimers of pancreatic ribonuclease.胰腺核糖核酸酶交联二聚体的制备
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Structural proteins of ribonucleic acid tumor viruses. Purification of envelope, core, and internal components.核糖核酸肿瘤病毒的结构蛋白。包膜、核心及内部成分的纯化。
J Biol Chem. 1976 Jan 25;251(2):559-64.
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Polyspermine-ribonuclease prepared by cross-linkage with dimethyl suberimidate.通过与辛二酸亚胺二甲酯交联制备的多精胺核糖核酸酶。
Biochemistry. 1977 Jun 28;16(13):2937-42. doi: 10.1021/bi00632a021.
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Ribonucleases and neoplasia.
Subcell Biochem. 1975 Sep;4(3):185-211.
8
Effect of RNA tumor virus-specific protein p30 on reverse transcriptase. Intraspecies and interspecies interaction between reverse transcriptase and p30.RNA肿瘤病毒特异性蛋白p30对逆转录酶的作用。逆转录酶与p30的种内和种间相互作用。
J Biol Chem. 1978 Nov 25;253(22):8285-90.
9
Complexing Rauscher leukemia virus reverse transcriptase with human plasma ribonuclease from Hodgkin's disease patients.使劳氏肉瘤病毒逆转录酶与霍奇金病患者的人血浆核糖核酸酶形成复合物。
J Biol Chem. 1977 Nov 10;252(21):7783-7.
10
Effect of Rauscher leukemia virus-specific proteins on reverse transcriptase. Binding between reverse transcriptase and p30.劳氏肉瘤病毒特异性蛋白对逆转录酶的作用。逆转录酶与p30之间的结合。
J Biol Chem. 1977 Aug 25;252(16):5883-7.

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