受刺激的正常人淋巴细胞含有一种对核糖核酸酶敏感的DNA聚合酶，它不同于病毒RNA指导的DNA聚合酶。
Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.
作者信息
Bobrow S N, Smith R G, Reitz M S, Gallo R C
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3228-32. doi: 10.1073/pnas.69.11.3228.
Abstract
Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes. DNA polymerase purified from this fraction does not transcribe the heteropolymeric regions of 70S RNA from RNA tumor viruses, thus distinguishing this enzyme from the RNA-directed DNA polymerase (reverse transcriptase) found in oncogenic RNA viruses and human leukemic cells.
摘要
在用植物血凝素刺激的正常人血淋巴细胞的细胞质颗粒部分中，可证明核糖核酸酶敏感的DNA合成，但在未刺激的淋巴细胞中则未观察到。从该部分纯化的DNA聚合酶不会转录RNA肿瘤病毒70S RNA的杂聚区域，因此将这种酶与致癌RNA病毒和人类白血病细胞中发现的RNA指导的DNA聚合酶（逆转录酶）区分开来。
相似文献
1
Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3228-32. doi: 10.1073/pnas.69.11.3228.
2
Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates.
Science. 1972 May 19;176(4036):798-800. doi: 10.1126/science.176.4036.798.
3
dsDNA made by RNase-sensitive DNA polymerase from RSV-transformed cells.
Nature. 1974 May 31;249(456):441-5. doi: 10.1038/249441a0.
4
DNA-direced and RNA-primed DNA synthesis in microsomal and mitochondrial fractions of normal human lymphocytes.
Biochem Biophys Res Commun. 1974 Apr 8;57(3):934-48. doi: 10.1016/0006-291x(74)90635-4.
5
[DNA polymerases of RNA tumor virus (RNA-DNA reaction)].
Tanpakushitsu Kakusan Koso. 1973 Jan;18(1):18-34.
6
Reverse transcriptase-progress, problems and prospects.
Bibl Haematol. 1973;39:1165-81.
7
Avian myeloblastosis virus DNA polymerase: initiation of DNA synthesis and an associated ribonuclease.
Johns Hopkins Med J Suppl. 1973;2:193-8.
8
Endogenous RNA-directed DNA polymerase activity in normal cells.
Johns Hopkins Med J Suppl. 1973;2:141-56.
9
Terminal riboadenylate transferase in human lymphocytes.
Nature. 1974 Mar 29;248(447):407-9. doi: 10.1038/248407a0.
10
Copying natural RNAs with E. coli DNA polymerase I.
Nat New Biol. 1973 Mar 21;242(116):66-9. doi: 10.1038/newbio242066a0.
引用本文的文献
1
The Assembly of HTLV-1-How Does It Differ from HIV-1?
Viruses. 2024 Sep 27;16(10):1528. doi: 10.3390/v16101528.
2
Reflections on Some of the Exceptional Features of HTLV-1 and HTLV-1 Research: A Perspective.
Front Immunol. 2022 Apr 1;13:859654. doi: 10.3389/fimmu.2022.859654. eCollection 2022.
3
The discovery of HTLV-1, the first pathogenic human retrovirus.
Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):15525-9. doi: 10.1073/pnas.1521629112.
4
A historical reflection on the discovery of human retroviruses.
Retrovirology. 2009 May 1;6:40. doi: 10.1186/1742-4690-6-40.
5
The discovery of the first human retrovirus: HTLV-1 and HTLV-2.
Retrovirology. 2005 Mar 2;2:17. doi: 10.1186/1742-4690-2-17.
6
Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes.
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1887-91. doi: 10.1073/pnas.78.3.1887.
7
A diploid rat liver cell culture. 2. Effect of aflatoxin B1 on ribonucleic acid synthesis.
In Vitro. 1974 Jan-Feb;9(4):294-301. doi: 10.1007/BF02616076.
8
Reverse transcriptase activity: increase in marrow cultures from leukaemic patients in relapse and remission.
Br J Cancer. 1974 Jun;29(6):433-7. doi: 10.1038/bjc.1974.95.
9
[Biochemical differences of DNA-polymerases in leukemic cells].
Blut. 1974 Apr;28(4):256-63. doi: 10.1007/BF01631646.
10
Purification, characterization, and comparison of the DNA polymerases from two primate RNA tumor viruses.
J Virol. 1973 Sep;12(3):431-9. doi: 10.1128/JVI.12.3.431-439.1973.
本文引用的文献
1
RNA dependent DNA synthesis in cell free preparations of human leukemia cells.
Biochem Biophys Res Commun. 1971 Feb 19;42(4):723-9. doi: 10.1016/0006-291x(71)90547-x.
2
Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids.
Eur J Biochem. 1970 Jun;14(2):278-83. doi: 10.1111/j.1432-1033.1970.tb00287.x.
3
Synthesis of amplified DNA that codes for ribosomal RNA.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2769-73. doi: 10.1073/pnas.68.11.2769.
4
RNA dependent DNA polymerase of human acute leukaemic cells.
Nature. 1970 Dec 5;228(5275):927-9. doi: 10.1038/228927a0.
5
A chemical model for transcriptional initiation of DNA replication.
Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354-60. doi: 10.1016/s0006-291x(72)80124-4.
6
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9. doi: 10.1073/pnas.68.11.2826.
7
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9. doi: 10.1073/pnas.69.4.965.
8
An RNA-DNA complex intermediate in ribosomal gene amplification.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1749-52. doi: 10.1073/pnas.69.7.1749.
9
On the role of RNA in gene amplification.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1746-8. doi: 10.1073/pnas.69.7.1746.
10
RNA-primed DNA synthesis in vitro.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1560-4. doi: 10.1073/pnas.69.6.1560.
Zotero 插件