受刺激的正常人淋巴细胞含有一种对核糖核酸酶敏感的DNA聚合酶，它不同于病毒RNA指导的DNA聚合酶。
Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.
作者信息
Bobrow S N, Smith R G, Reitz M S, Gallo R C
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3228-32. doi: 10.1073/pnas.69.11.3228.
Abstract
Ribonuclease-sensitive DNA synthesis is demonstrated in a cytoplasmic particulate fraction of normal human blood lymphocytes stimulated with phytohemagglutinin, but not in unstimulated lymphocytes. DNA polymerase purified from this fraction does not transcribe the heteropolymeric regions of 70S RNA from RNA tumor viruses, thus distinguishing this enzyme from the RNA-directed DNA polymerase (reverse transcriptase) found in oncogenic RNA viruses and human leukemic cells.
摘要
在用植物血凝素刺激的正常人血淋巴细胞的细胞质颗粒部分中，可证明核糖核酸酶敏感的DNA合成，但在未刺激的淋巴细胞中则未观察到。从该部分纯化的DNA聚合酶不会转录RNA肿瘤病毒70S RNA的杂聚区域，因此将这种酶与致癌RNA病毒和人类白血病细胞中发现的RNA指导的DNA聚合酶（逆转录酶）区分开来。
相似文献
1
Stimulated normal human lymphocytes contain a ribonuclease-sensitive DNA polymerase distinct from viral RNA-directed DNA polymerase.受刺激的正常人淋巴细胞含有一种对核糖核酸酶敏感的DNA聚合酶，它不同于病毒RNA指导的DNA聚合酶。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3228-32. doi: 10.1073/pnas.69.11.3228.
2
Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates.病毒和细胞DNA聚合酶：与合成及天然RNA模板的活性比较
Science. 1972 May 19;176(4036):798-800. doi: 10.1126/science.176.4036.798.
3
dsDNA made by RNase-sensitive DNA polymerase from RSV-transformed cells.由来自劳氏肉瘤病毒转化细胞的核糖核酸酶敏感型DNA聚合酶合成的双链DNA。
Nature. 1974 May 31;249(456):441-5. doi: 10.1038/249441a0.
4
DNA-direced and RNA-primed DNA synthesis in microsomal and mitochondrial fractions of normal human lymphocytes.正常人淋巴细胞微粒体和线粒体组分中DNA指导及RNA引发的DNA合成
Biochem Biophys Res Commun. 1974 Apr 8;57(3):934-48. doi: 10.1016/0006-291x(74)90635-4.
5
[DNA polymerases of RNA tumor virus (RNA-DNA reaction)].[RNA肿瘤病毒的DNA聚合酶（RNA- DNA反应）]
Tanpakushitsu Kakusan Koso. 1973 Jan;18(1):18-34.
6
Reverse transcriptase-progress, problems and prospects.逆转录酶——进展、问题与前景
Bibl Haematol. 1973;39:1165-81.
7
Avian myeloblastosis virus DNA polymerase: initiation of DNA synthesis and an associated ribonuclease.禽成髓细胞瘤病毒DNA聚合酶：DNA合成的起始及一种相关的核糖核酸酶
Johns Hopkins Med J Suppl. 1973;2:193-8.
8
Endogenous RNA-directed DNA polymerase activity in normal cells.正常细胞中的内源性RNA指导的DNA聚合酶活性。
Johns Hopkins Med J Suppl. 1973;2:141-56.
9
Terminal riboadenylate transferase in human lymphocytes.人类淋巴细胞中的末端核糖腺苷酸转移酶
Nature. 1974 Mar 29;248(447):407-9. doi: 10.1038/248407a0.
10
Copying natural RNAs with E. coli DNA polymerase I.使用大肠杆菌DNA聚合酶I复制天然RNA。
Nat New Biol. 1973 Mar 21;242(116):66-9. doi: 10.1038/newbio242066a0.
引用本文的文献
1
The Assembly of HTLV-1-How Does It Differ from HIV-1?HTLV-1 衣壳的组装——它与 HIV-1 有何不同？
Viruses. 2024 Sep 27;16(10):1528. doi: 10.3390/v16101528.
2
Reflections on Some of the Exceptional Features of HTLV-1 and HTLV-1 Research: A Perspective.对 HTLV-1 和 HTLV-1 研究的一些特殊特征的思考：一个视角。
Front Immunol. 2022 Apr 1;13:859654. doi: 10.3389/fimmu.2022.859654. eCollection 2022.
3
The discovery of HTLV-1, the first pathogenic human retrovirus.人类嗜T淋巴细胞病毒1型（HTLV-1）的发现，这是首个致病性人类逆转录病毒。
Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):15525-9. doi: 10.1073/pnas.1521629112.
4
A historical reflection on the discovery of human retroviruses.对人类逆转录病毒发现的历史反思。
Retrovirology. 2009 May 1;6:40. doi: 10.1186/1742-4690-6-40.
5
The discovery of the first human retrovirus: HTLV-1 and HTLV-2.第一种人类逆转录病毒的发现：人类嗜T淋巴细胞病毒1型（HTLV-1）和人类嗜T淋巴细胞病毒2型（HTLV-2）。
Retrovirology. 2005 Mar 2;2:17. doi: 10.1186/1742-4690-2-17.
6
Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes.从人类肿瘤性T淋巴细胞中分离出的一种新型C型逆转录病毒核酸序列的特征及分布
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1887-91. doi: 10.1073/pnas.78.3.1887.
7
A diploid rat liver cell culture. 2. Effect of aflatoxin B1 on ribonucleic acid synthesis.一种二倍体大鼠肝细胞培养物。2. 黄曲霉毒素B1对核糖核酸合成的影响。
In Vitro. 1974 Jan-Feb;9(4):294-301. doi: 10.1007/BF02616076.
8
Reverse transcriptase activity: increase in marrow cultures from leukaemic patients in relapse and remission.逆转录酶活性：白血病复发和缓解患者骨髓培养物中的活性增加。
Br J Cancer. 1974 Jun;29(6):433-7. doi: 10.1038/bjc.1974.95.
9
[Biochemical differences of DNA-polymerases in leukemic cells].[白血病细胞中DNA聚合酶的生化差异]
Blut. 1974 Apr;28(4):256-63. doi: 10.1007/BF01631646.
10
Purification, characterization, and comparison of the DNA polymerases from two primate RNA tumor viruses.两种灵长类RNA肿瘤病毒DNA聚合酶的纯化、特性鉴定及比较
J Virol. 1973 Sep;12(3):431-9. doi: 10.1128/JVI.12.3.431-439.1973.
本文引用的文献
1
RNA dependent DNA synthesis in cell free preparations of human leukemia cells.人白血病细胞无细胞制剂中的RNA依赖性DNA合成
Biochem Biophys Res Commun. 1971 Feb 19;42(4):723-9. doi: 10.1016/0006-291x(71)90547-x.
2
Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids.核糖核酸酶H。一种降解DNA-RNA杂交体中RNA部分的酶。
Eur J Biochem. 1970 Jun;14(2):278-83. doi: 10.1111/j.1432-1033.1970.tb00287.x.
3
Synthesis of amplified DNA that codes for ribosomal RNA.编码核糖体RNA的扩增DNA的合成。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2769-73. doi: 10.1073/pnas.68.11.2769.
4
RNA dependent DNA polymerase of human acute leukaemic cells.人类急性白血病细胞的RNA依赖性DNA聚合酶
Nature. 1970 Dec 5;228(5275):927-9. doi: 10.1038/228927a0.
5
A chemical model for transcriptional initiation of DNA replication.DNA复制转录起始的化学模型。
Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354-60. doi: 10.1016/s0006-291x(72)80124-4.
6
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.RNA聚合酶在M13 DNA合成起始过程中的可能作用。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9. doi: 10.1073/pnas.68.11.2826.
7
RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.RNA合成引发M13 DNA在体外转化为其复制形式。
Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9. doi: 10.1073/pnas.69.4.965.
8
An RNA-DNA complex intermediate in ribosomal gene amplification.核糖体基因扩增过程中的一种RNA-DNA复合中间体。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1749-52. doi: 10.1073/pnas.69.7.1749.
9
On the role of RNA in gene amplification.论RNA在基因扩增中的作用。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1746-8. doi: 10.1073/pnas.69.7.1746.
10
RNA-primed DNA synthesis in vitro.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1560-4. doi: 10.1073/pnas.69.6.1560.
Zotero 插件