Apolipoprotein B (Apo B) in vein graft atherosclerosis. An immunoperoxidase study.

An immunoperoxidase procedure was used to localize the apolipoprotein B in the atherosclerotic lesions of vein grafts removed 5 years after an aortocoronary bypass operation and preserved by embedding in paraffin. Apolipoprotein B was mainly found at the cell-rich interface of the lesion proper and the underlying fibrous tissue. The findings of this study indicate that the immunoperoxidase procedure can be used to investigate the localization of immunoactive components in paraffin-embedded tissue sections.