Pinkel D
Environ Health Perspect. 1981 Jan;37:133-6. doi: 10.1289/ehp.8137133.
Both flow cytometry and automated image analysis techniques may be useful in relieving the drudgery, increasing the speed, and adding quantitation to the scoring of pollen mutants, but practical problems related to the detection of very rare events have to be overcome. The features of flow and image cytometry instrumentation that may be useful for pollen measurements are discussed and a qualitative framework is presented from which to view the rare event problem.
流式细胞术和自动图像分析技术在减轻花粉突变体评分的繁重工作、提高速度以及增加定量方面都可能有用,但必须克服与检测极罕见事件相关的实际问题。讨论了可能对花粉测量有用的流式细胞术和图像细胞术仪器的特点,并提出了一个定性框架,用以审视罕见事件问题。