一种针对人甲胎蛋白的单克隆杂交瘤抗体的衍生与特性研究

Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein.

作者信息

Tsung Y K, Milunsky A, Alpert E

出版信息

J Immunol Methods. 1980;39(4):363-8. doi: 10.1016/0022-1759(80)90236-7.

PMID:6161969

Abstract

A monoclonal antibody specific for human alpha-fetoprotein (HAFP) was derived by the hybridoma technique. Spleen cells from mice immunized with pure HAFP were fused with a mouse myeloma cell line (P3x63-Ag-8) and an anti-HAFP secreting hybridoma cell line was cloned in soft agarose. The HAFP specific antibody was shown to be a monoclonal IgG1 subclass with extremely high avidity for HAFP.

摘要

通过杂交瘤技术获得了一种针对人甲胎蛋白（HAFP）的单克隆抗体。用纯HAFP免疫的小鼠的脾细胞与小鼠骨髓瘤细胞系（P3x63-Ag-8）融合，并在软琼脂糖中克隆出分泌抗HAFP的杂交瘤细胞系。已证明该HAFP特异性抗体是一种单克隆IgG1亚类，对HAFP具有极高的亲和力。