小鼠克隆核糖体RNA基因推定转录起始位点的核苷酸序列。

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

作者信息

Urano Y, Kominami R, Mishima Y, Muramatsu M

出版信息

Nucleic Acids Res. 1980 Dec 20;8(24):6043-58. doi: 10.1093/nar/8.24.6043.

DOI:10.1093/nar/8.24.6043

PMID:6162156

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328071/

Abstract

Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site was determined by two independent methods: one nuclease S1 protection and the other reverse transcriptase elongation mapping using isolated 45S ribosomal RNA precursor (45S RNA) and appropriate restriction fragments of rDNA. Both methods gave an identical result; 45S RNA had a structure starting from ACTCTTAG---. Characteristically, mouse rDNA had many T clusters (greater than or equal to 5) upstream the initiation site, the longest being 21 consecutive T's. A pentadecanucleotide, TGCCTCCCGAGTGCA, appeared twice within 260 nucleotides upstream the putative initiation site. No such characteristic sequences were found downstream this site. Little similarity was found in the upstream of the transcription initiation site between the mouse, Xenopus laevis and Saccharomyces cerevisiae rDNA.

摘要

对小鼠克隆核糖体DNA（rDNA）转录起始位点周围及上游约1千碱基对进行了测序。通过两种独立方法确定了推定的转录起始位点：一种是核酸酶S1保护法，另一种是使用分离的45S核糖体RNA前体（45S RNA）和rDNA的适当限制性片段进行逆转录酶延伸图谱分析。两种方法得到了相同的结果；45S RNA具有从ACTCTTAG---开始的结构。其特征是，小鼠rDNA在起始位点上游有许多T簇（大于或等于5个），最长的是21个连续的T。一个十五聚体TGCCTCCCGAGTGCA在推定起始位点上游260个核苷酸内出现了两次。在该位点下游未发现此类特征序列。在小鼠、非洲爪蟾和酿酒酵母rDNA转录起始位点的上游几乎没有发现相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c1/328071/3dd7dc1c0702/nar00441-0126-a.jpg

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小鼠克隆核糖体RNA基因推定转录起始位点的核苷酸序列。

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

作者信息

Urano Y, Kominami R, Mishima Y, Muramatsu M

出版信息

Nucleic Acids Res. 1980 Dec 20;8(24):6043-58. doi: 10.1093/nar/8.24.6043.

DOI:10.1093/nar/8.24.6043

PMID:6162156

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328071/

Abstract

摘要

小鼠克隆核糖体RNA基因推定转录起始位点的核苷酸序列。

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

作者信息

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小鼠克隆核糖体RNA基因推定转录起始位点的核苷酸序列。

The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.

作者信息

出版信息

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