大鼠核糖体DNA的转录起始位点。
Transcription initiation site of rat ribosomal DNA.
作者信息
Rothblum L I, Reddy R, Cassidy B
出版信息
Nucleic Acids Res. 1982 Nov 25;10(22):7345-62. doi: 10.1093/nar/10.22.7345.
The sequence of 1,100 nucleotides surrounding the transcription initiation site of a cloned rat ribosomal RNA gene (rDNA) has been determined. The location of the 5' terminus of 45S pre-rRNA was determined by S1 nuclease mapping, reverse transcriptase elongation and confirmed by in vitro capping of 45S rRNA and in vitro transcription. Two different plasmid subclones, from two separate genomic clones of rat rDNA, contained the identical sequence surrounding the transcription initiation site: -10GGAGATATAT 1GCTGACACGC TGTCCTTTTG+20. Relatively long, greater than 15 base pairs, regions of sequence homology were found when the sequences of the initiation regions of rat and mouse rDNA (Urano, Y., Kominami, R., Mishima, Y., and Muramatsu, M., Nucleic Acids Res. 8, 6043-6058, 1980) were compared. When both the rat and mouse sequences were compared to that of human rDNA (G. Wilson, personal communication) a sequence of 15 nucleotides immediately following the initiation sites were found to be preserved.
已确定了克隆的大鼠核糖体RNA基因（rDNA）转录起始位点周围1100个核苷酸的序列。通过S1核酸酶图谱分析、逆转录酶延伸法确定了45S前体rRNA 5'末端的位置，并通过45S rRNA的体外加帽和体外转录得到了证实。来自大鼠rDNA两个不同基因组克隆的两种不同质粒亚克隆，在转录起始位点周围包含相同的序列：-10GGAGATATAT 1GCTGACACGC TGTCCTTTTG+20。当比较大鼠和小鼠rDNA起始区域的序列时（Urano, Y., Kominami, R., Mishima, Y., and Muramatsu, M., Nucleic Acids Res. 8, 6043-6058, 1980），发现了相对较长（大于15个碱基对）的序列同源区域。当将大鼠和小鼠的序列与人rDNA的序列进行比较时（G. Wilson, 个人交流），发现在起始位点之后紧接着有15个核苷酸的序列得以保留。
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