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大鼠核糖体DNA的转录起始位点。

Transcription initiation site of rat ribosomal DNA.

作者信息

Rothblum L I, Reddy R, Cassidy B

出版信息

Nucleic Acids Res. 1982 Nov 25;10(22):7345-62. doi: 10.1093/nar/10.22.7345.

DOI:10.1093/nar/10.22.7345
PMID:6296773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327008/
Abstract

The sequence of 1,100 nucleotides surrounding the transcription initiation site of a cloned rat ribosomal RNA gene (rDNA) has been determined. The location of the 5' terminus of 45S pre-rRNA was determined by S1 nuclease mapping, reverse transcriptase elongation and confirmed by in vitro capping of 45S rRNA and in vitro transcription. Two different plasmid subclones, from two separate genomic clones of rat rDNA, contained the identical sequence surrounding the transcription initiation site: -10GGAGATATAT 1GCTGACACGC TGTCCTTTTG+20. Relatively long, greater than 15 base pairs, regions of sequence homology were found when the sequences of the initiation regions of rat and mouse rDNA (Urano, Y., Kominami, R., Mishima, Y., and Muramatsu, M., Nucleic Acids Res. 8, 6043-6058, 1980) were compared. When both the rat and mouse sequences were compared to that of human rDNA (G. Wilson, personal communication) a sequence of 15 nucleotides immediately following the initiation sites were found to be preserved.

摘要

已确定了克隆的大鼠核糖体RNA基因（rDNA）转录起始位点周围1100个核苷酸的序列。通过S1核酸酶图谱分析、逆转录酶延伸法确定了45S前体rRNA 5'末端的位置，并通过45S rRNA的体外加帽和体外转录得到了证实。来自大鼠rDNA两个不同基因组克隆的两种不同质粒亚克隆，在转录起始位点周围包含相同的序列：-10GGAGATATAT 1GCTGACACGC TGTCCTTTTG+20。当比较大鼠和小鼠rDNA起始区域的序列时（Urano, Y., Kominami, R., Mishima, Y., and Muramatsu, M., Nucleic Acids Res. 8, 6043-6058, 1980），发现了相对较长（大于15个碱基对）的序列同源区域。当将大鼠和小鼠的序列与人rDNA的序列进行比较时（G. Wilson, 个人交流），发现在起始位点之后紧接着有15个核苷酸的序列得以保留。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/bd217927c8a3/nar00391-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/a3ead0ff7c86/nar00391-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/aa88e02d6bae/nar00391-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/44cf44e25d4f/nar00391-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/a3fcf25836fd/nar00391-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/38cf66bf82eb/nar00391-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/bd217927c8a3/nar00391-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/a3ead0ff7c86/nar00391-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/aa88e02d6bae/nar00391-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/44cf44e25d4f/nar00391-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/a3fcf25836fd/nar00391-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/38cf66bf82eb/nar00391-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df6f/327008/bd217927c8a3/nar00391-0303-a.jpg

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本文引用的文献

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Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.RNA聚合酶I对小鼠rRNA基因的转录：体外和体内的起始及加工位点
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Ribosomal RNA transcription in vitro is species specific.体外核糖体RNA转录具有物种特异性。
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The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.酵母核糖体RNA基因的结构。2. 核糖体RNA转录起始位点的核苷酸序列。
Ribin, a protein encoded by a message complementary to rRNA, modulates ribosomal transcription and cell proliferation.
核糖蛋白（Ribin）是一种由与核糖体RNA互补的信使RNA编码的蛋白质，可调节核糖体转录和细胞增殖。
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Structure of recombinant rat UBF by electron image analysis and homology modelling.通过电子图像分析和同源建模解析重组大鼠UBF的结构。
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The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor.物种特异性的RNA聚合酶I转录因子SL-1与上游结合因子结合。
Mol Cell Biol. 1996 Feb;16(2):557-63. doi: 10.1128/MCB.16.2.557.
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Coordinated decreases in rRNA gene transcription factors and rRNA synthesis during muscle cell differentiation.肌肉细胞分化过程中rRNA基因转录因子和rRNA合成的协同减少。
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7933-6. doi: 10.1073/pnas.90.17.7933.
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Enhancer 1 binding factor, a Ku-related protein, is a positive regulator of RNA polymerase I transcription initiation.
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E1BF/Ku interacts physically and functionally with the core promoter binding factor CPBF and promotes the basal transcription of rat and human ribosomal RNA genes.E1BF/Ku与核心启动子结合因子CPBF在物理和功能上相互作用，并促进大鼠和人类核糖体RNA基因的基础转录。
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Activation of RNA polymerase III transcription of human Alu repetitive elements by adenovirus type 5: requirement for the E1b 58-kilodalton protein and the products of E4 open reading frames 3 and 6.5型腺病毒对人Alu重复元件RNA聚合酶III转录的激活作用：对E1b 58千道尔顿蛋白以及E4开放阅读框3和6产物的需求
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A purified fraction containing RNA polymerase I that can accurately transcribe rat ribosomal RNA gene.一种含有RNA聚合酶I的纯化组分，其能够准确转录大鼠核糖体RNA基因。
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Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.人类核糖体RNA基因：转录起始区域的核苷酸序列及三种哺乳动物基因的比较
Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6. doi: 10.1073/pnas.79.10.3092.
5
Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA gene.大鼠核糖体RNA基因转录起始区域的核苷酸序列。
Gene. 1982 May;18(2):115-22. doi: 10.1016/0378-1119(82)90109-3.
6
Nucleotide sequence of the region between the 18S rRNA sequence and the 28S rRNA sequence of rat ribosomal DNA.大鼠核糖体DNA的18S rRNA序列与28S rRNA序列之间区域的核苷酸序列。
Nucleic Acids Res. 1982 Jun 25;10(12):3667-80. doi: 10.1093/nar/10.12.3667.
7
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Gene. 1982 Jan;17(1):75-7. doi: 10.1016/0378-1119(82)90102-0.
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Nucleic Acids Res. 1981 Nov 25;9(22):6093-102. doi: 10.1093/nar/9.22.6093.
9
Structure of the Tetrahymena pyriformis rRNA gene. Nucleotide sequence of the transcription initiation region.梨形四膜虫rRNA基因的结构。转录起始区域的核苷酸序列。
J Biol Chem. 1981 Dec 25;256(24):12849-56.
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Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.黑腹果蝇核糖体RNA转录起始位点的核苷酸序列：有插入和无插入基因的比较。
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