Whitacre L R, Nathan P
Tissue Antigens. 1980 Nov;16(5):393-8. doi: 10.1111/j.1399-0039.1980.tb00320.x.
Lymphocyte or platelet absorption of multispecific sera is frequently used to establish HLA antibody specificity. Absorption of such sera with cells containing the desired antigenic combinations is often limited by the availability and the HLA characteristics of fresh or frozen cell panels. The method described permits improved absorption of specific HLA antibody with a preparation of cells containing a predetermined single HLA antigen. The procedure involves the screening of the patient's active sera against a panel of frozen cells. Analysis of the positive and negative reactions allows tentative assessment of recipient serum specificities. Cells with a single available HLA antigen type are prepared for serum absorption by suspending the cells in selected antisera to block (mask) HLA surface antigens while leaving just one type of antigen free for monospecific absorption. Following absorption of the test serum with the masked cells, the absorbed serum is then retested against the original panel of frozen cells. Should the results of this screen show a loss of a single HLA activity, the suspected specificity is assigned to the recipient's serum. The use of HLA antigen-masked lymphocytes has proven useful in the definition of HLA antibody specificity.
多特异性血清的淋巴细胞或血小板吸收常用于确定HLA抗体特异性。用含有所需抗原组合的细胞吸收此类血清，常常受到新鲜或冷冻细胞板的可用性和HLA特征的限制。所描述的方法允许用含有预定单一HLA抗原的细胞制剂更好地吸收特异性HLA抗体。该程序包括用一组冷冻细胞筛选患者的活性血清。对阳性和阴性反应的分析有助于初步评估受者血清的特异性。通过将细胞悬浮在选定的抗血清中以阻断（掩盖）HLA表面抗原，同时仅留下一种类型的抗原用于单特异性吸收，制备具有单一可用HLA抗原类型的细胞用于血清吸收。在用掩盖细胞吸收测试血清后，然后将吸收后的血清针对原始冷冻细胞板重新进行检测。如果该筛选结果显示单一HLA活性丧失，则将可疑特异性归因于受者血清。已证明使用HLA抗原掩盖的淋巴细胞有助于定义HLA抗体特异性。
