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兔网织红细胞裂解物中与干扰素处理细胞中的激酶类似的双链RNA依赖性蛋白激酶。

Double-stranded RNA dependent protein kinase (s) in rabbit reticulocyte lysates analogous to kinase from interferon-treated cells.

作者信息

Hovanessian A G

出版信息

Biochimie. 1980;62(11-12):775-8. doi: 10.1016/s0300-9084(80)80132-5.

DOI:10.1016/s0300-9084(80)80132-5
PMID:6162487
Abstract

A double-stranded (ds) RNA-dependent protein kinase(s) from haemin-supplemented rabbit reticulocyte lysates in purified by chromatography on columns of poly(I).poly(C)-Sepharose. This method provides a more than 1000-fold purifications of the kinase(s) in a single step and a high yield. The purified kinase is responsible for the phosphorylation of (a) a polypeptide of molecular weight 67000, (b) the smallest subunit eIF-2 and (c) added histones. These kinase activities are comparable to the dsRNA-dependent protein kinase activities from interferon-treated cells.

摘要

通过在聚（I）·聚（C）-琼脂糖柱上进行层析，从添加了血红素的兔网织红细胞裂解物中纯化出一种双链（ds）RNA依赖性蛋白激酶。该方法可在一步操作中实现激酶超过1000倍的纯化，且产率高。纯化后的激酶负责使（a）分子量为67000的多肽、（b）最小亚基eIF-2以及（c）添加的组蛋白发生磷酸化。这些激酶活性与经干扰素处理的细胞中的dsRNA依赖性蛋白激酶活性相当。

相似文献

1
Double-stranded RNA dependent protein kinase (s) in rabbit reticulocyte lysates analogous to kinase from interferon-treated cells.兔网织红细胞裂解物中与干扰素处理细胞中的激酶类似的双链RNA依赖性蛋白激酶。
Biochimie. 1980;62(11-12):775-8. doi: 10.1016/s0300-9084(80)80132-5.
2
Purification and properties of the double-stranded RNA-activated eukaryotic initiation factor 3 kinase from rabbit reticulocytes.兔网织红细胞双链RNA激活的真核起始因子3激酶的纯化及性质
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6526-30. doi: 10.1073/pnas.77.11.6526.
3
Characterization of double-stranded-RNA-activated kinase that phosphorylates alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) in reticulocyte lysates.网织红细胞裂解物中使真核起始因子2（eIF-2α）的α亚基磷酸化的双链RNA激活激酶的特性分析
Proc Natl Acad Sci U S A. 1980 Feb;77(2):832-6. doi: 10.1073/pnas.77.2.832.
4
Activation of the double-stranded RNA-dependent eIF-2 alpha kinase by cellular RNA from 3T3-F442A cells.来自3T3-F442A细胞的细胞RNA对双链RNA依赖性eIF-2α激酶的激活作用。
Eur J Biochem. 1991 Jan 1;195(1):41-8. doi: 10.1111/j.1432-1033.1991.tb15673.x.
5
Double-stranded RNA-dependent eIF-2alpha protein kinase.
Methods Enzymol. 1983;99:346-62. doi: 10.1016/0076-6879(83)99070-5.
6
Double-stranded RNA-dependent phosphorylation of protein P1 and eukaryotic initiation factor 2 alpha does not correlate with protein synthesis inhibition in a cell-free system from interferon-treated mouse L cells.在经干扰素处理的小鼠L细胞的无细胞体系中，蛋白质P1和真核起始因子2α的双链RNA依赖性磷酸化与蛋白质合成抑制不相关。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):41-5. doi: 10.1073/pnas.80.1.41.
7
Regulation of double-stranded RNA-activated eukaryotic initiation factor 2 alpha kinase by type 2 protein phosphatase in reticulocyte lysates.网织红细胞裂解物中2型蛋白磷酸酶对双链RNA激活的真核起始因子2α激酶的调节
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6512-6. doi: 10.1073/pnas.79.21.6512.
8
Mechanism of interferon action. Purification and substrate specificities of the double-stranded RNA-dependent protein kinase from untreated and interferon-treated mouse fibroblasts.干扰素作用机制。未处理及经干扰素处理的小鼠成纤维细胞中双链RNA依赖性蛋白激酶的纯化及底物特异性
J Biol Chem. 1985 Sep 15;260(20):11240-7.
9
Synthesis of an oligonucleotide inhibitor of protein synthesis in rabbit reticulocyte lysates analogous to that formed in extracts from interferon-treated cells.在兔网织红细胞裂解物中合成一种蛋白质合成的寡核苷酸抑制剂，类似于在干扰素处理细胞提取物中形成的抑制剂。
Eur J Biochem. 1978 Mar;84(1):149-59. doi: 10.1111/j.1432-1033.1978.tb12151.x.
10
Control of protein synthesis in human reticulocytes by heme-regulated and double-stranded RNA dependent eIF-2 alpha kinases.血红素调节的和双链RNA依赖性的真核起始因子2α激酶对人网织红细胞中蛋白质合成的调控
Biochem Biophys Res Commun. 1984 Mar 30;119(3):891-9. doi: 10.1016/0006-291x(84)90857-x.

引用本文的文献

1
The B56alpha regulatory subunit of protein phosphatase 2A is a target for regulation by double-stranded RNA-dependent protein kinase PKR.蛋白磷酸酶2A的B56α调节亚基是双链RNA依赖性蛋白激酶PKR的调节靶点。
Mol Cell Biol. 2000 Jul;20(14):5285-99. doi: 10.1128/MCB.20.14.5285-5299.2000.
2
Tumor suppressor function of the interferon-induced double-stranded RNA-activated protein kinase.干扰素诱导的双链RNA激活蛋白激酶的肿瘤抑制功能
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):232-6. doi: 10.1073/pnas.90.1.232.