胡宁病毒蛋白的生物学活性。II. 与可溶性抗原及纯化病毒颗粒相关的补体结合多肽。

Biological activities of Junin virus proteins. II. Complement-fixing polypeptides associated with the soluble antigen and purified virus particles.

作者信息

de Mitri M I, de Martinez Segovia Z M

出版信息

Intervirology. 1980;14(2):84-90. doi: 10.1159/000149167.

Abstract

In the Junin virus-infected cell, there is a soluble antigen (SAg), which we have detected by complement fixation. PAGE of purified SAg revealed two polypeptides with molecular weights of 20,000 and 25,000. An antigenically identical component, which was demonstrated by complement fixation and immunodiffusion tests and which had the same electrophoretic profile as SAg, was found after proteolytic breakdown of purified virions. SAg is an internal component of the virion, since it is unable to stimulate neutralizing antibodies and it can be obtained from viral particles whose envelopes have been removed by Triton X-100 treatment. The antigenic determinant apparently resides in the major nonglycosylated polypeptide (nucleoprotein P64), since the proteolytic degradation product is obtained in quantity and it is nonglycosylated.

摘要

在胡宁病毒感染的细胞中，存在一种可溶性抗原（SAg），我们已通过补体结合试验检测到它。纯化后的SAg经聚丙烯酰胺凝胶电泳显示出两条分子量分别为20,000和25,000的多肽。在纯化病毒粒子经蛋白水解后，发现了一种抗原性相同的成分，该成分经补体结合试验和免疫扩散试验证实，并且具有与SAg相同的电泳图谱。SAg是病毒粒子的一种内部成分，因为它不能刺激中和抗体产生，并且可以从经Triton X-100处理去除包膜的病毒颗粒中获得。抗原决定簇显然存在于主要的非糖基化多肽（核蛋白P64）中，因为蛋白水解降解产物大量产生且为非糖基化产物。