Sanchez A, Pifat D Y, Kenyon R H, Peters C J, McCormick J B, Kiley M P
Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Gen Virol. 1989 May;70 ( Pt 5):1125-32. doi: 10.1099/0022-1317-70-5-1125.
Twenty-one monoclonal antibodies reactive with Junin virus structural proteins were produced and characterized. Using radioimmunoprecipitation and Western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. In radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35K structural glycoprotein, but also with what is presumed to be the glycoprotein precursor. Four of seven glycoprotein-specific antibodies neutralized Junin virus to high titres. Cross-reactivity with other arenaviruses was found to be restricted to nucleoprotein-specific monoclonal antibodies and occurred only with New World arenaviruses. Cross-reactivity also shows the Junin virus to be most closely related to Machupo and Tacaribe viruses.
制备并鉴定了21种与胡宁病毒结构蛋白反应的单克隆抗体。通过放射免疫沉淀和蛋白质免疫印迹分析发现，其中13种与核蛋白反应，7种与表面糖蛋白反应，1种不发生反应，但显示出与其他糖蛋白特异性抗体一致的荧光抗体染色模式。在放射免疫沉淀分析中，糖蛋白特异性单克隆抗体不仅与35K结构糖蛋白反应，还与推测的糖蛋白前体反应。7种糖蛋白特异性抗体中有4种能高效中和胡宁病毒。发现与其他沙粒病毒的交叉反应仅限于核蛋白特异性单克隆抗体，且仅发生在新大陆沙粒病毒中。交叉反应还表明胡宁病毒与马丘波病毒和塔卡里贝病毒关系最为密切。
