Rogowski B A, Feng A S
J Comp Neurol. 1981 Feb 10;196(1):85-97. doi: 10.1002/cne.901960108.
Bipolar neurons in the medial superior olivary (MSO) nucleus of albino rats were studied at various ages (0-75 days) during development in order to elucidate normal neuronal growth patterns. Neurons, visualized in celloidin embedded Golgi/Nissl- and frozen Nissl-stained sections, were analyzed quantitatively for cell size and extent of dendritic arborization. These parameters, from both sets of data, showed a consistent developmental trend with the existence of two major stages in the ontogenetic process: a rapid developmental and a stabilization stage. The first stage, encompassing postnatal days 0 through approximately 14, was characterized by a rapidly increasing cell size, as observed in frozen sections. At the peak of this rapid growth, Golgi-stained neurons possessed thick dendrites with many appendages, growth cones, and filopodia. The peak in neuronal development coincided with the initiation of ear opening. Morphological features attained a more mature form in the second, stabilizing stage of development, which began at approximately postnatal day 14. This phase was distinguished by a decrement in cell size and dendritic branching. Also, dendrites possessed more regularly distributed varicosities and fewer appendages, growth cones, and filopodia in this period. It was observed that dendritic branching trends paralleled those of cell diameter growth.
为了阐明正常的神经元生长模式，研究了白化大鼠内侧上橄榄核（MSO）中不同年龄（0 - 75天）发育阶段的双极神经元。在火棉胶包埋的高尔基/尼氏染色切片和冷冻尼氏染色切片中观察神经元，并对细胞大小和树突分支范围进行定量分析。这两组数据的这些参数显示出一致的发育趋势，在个体发育过程中存在两个主要阶段：快速发育阶段和稳定阶段。第一阶段包括出生后0天至大约14天，其特征是细胞大小迅速增加，这在冷冻切片中可以观察到。在这种快速生长的高峰期，高尔基染色的神经元具有带有许多附属物、生长锥和丝状伪足的粗壮树突。神经元发育的高峰期与耳朵张开的开始时间一致。在大约出生后第14天开始的第二个稳定发育阶段，形态特征达到了更成熟的形式。这个阶段的特点是细胞大小和树突分支减少。此外，在此期间，树突具有分布更规则的膨体，附属物、生长锥和丝状伪足较少。观察到树突分支趋势与细胞直径生长趋势平行。
