Tojo H, Huston T M
J Reprod Fertil. 1981 Mar;61(2):381-6. doi: 10.1530/jrf.0.0610381.
The spontaneous ovulation of hens was suppressed by daily injection of PMSG. The LH injected to overcome the block was accompanied by one of 4 compounds known to inhibit steroidogenesis at different sites in the biosynthetic pathway. A dose of 300 mg aminoglutethimide phosphate, which inhibits the conversion of cholesterol to 20 alpha-hydroxycholesterol, blocked the LH-induced ovulation and prevented the normal rise in plasma progesterone. Metyrapone, an inhibitor of 11 beta-hydroxylase, and SC 12937 and AY 9944, inhibitors of cholesterol synthesis, did not prevent ovulation or the progesterone rise induced by exogenous LH. Administration of progesterone overcame the inhibitory effect of aminoglutethimide, and it is suggested that progesterone is involved in the ovulatory process of the fowl.
通过每日注射孕马血清促性腺激素（PMSG）抑制母鸡的自发排卵。为克服这种阻断而注射的促黄体生成素（LH）与已知在生物合成途径中不同位点抑制类固醇生成的4种化合物之一同时使用。剂量为300 mg的磷酸氨基乙腈，它抑制胆固醇向20α-羟基胆固醇的转化，阻断了LH诱导的排卵，并阻止了血浆孕酮的正常升高。11β-羟化酶抑制剂甲吡酮，以及胆固醇合成抑制剂SC 12937和AY 9944，并未阻止外源LH诱导的排卵或孕酮升高。给予孕酮可克服氨基乙腈的抑制作用，提示孕酮参与家禽的排卵过程。
