[使用蛋白质膜进行定量细胞光度测定的模型实验（作者译）]
[Model experiments for quantitative cytophotometry using protein films (author's transl)].
作者信息
Desoye G, Schauenstein E
出版信息
Microsc Acta. 1981 Jan;84(1):9-16.
PMID:6163067
Abstract
The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.
摘要
研究了用乙醇/乙醚变性和固定的蛋白质膜作为细胞化学模型系统的实用性。分析了用酰胺黑10B染色的动力学以及蛋白质-染料复合物的光谱性质。估计了该复合物的吸收系数。将目前获得的数据与染色细胞和溶液中的蛋白质-染料复合物进行了比较。讨论的重点是固定的影响以及将这些膜用作校准细胞化学染色系统的可能性。
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