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[利用染料结合法对溶液中的蛋白质进行定量测定(综述)]

[Use of dye binding for quantitative determination of proteins in solutions (review)].

作者信息

Shishkin S S

出版信息

Vopr Med Khim. 1982 Sep-Oct;28(5):134-41.

PMID:6758339
Abstract

A review is presented of the methods used for estimation of protein content in solutions. These methods are based on measuring of some organic dyes binding with protein--amido-black 10B, coomassy brilliant blue R-250 and G-250, ponso G, violet sulfate, bromphenol blue, naphthol yellow S, orange G. Advantages and limitations of these methods were compared with Lowry et al (1951) method and with some other conventional methods for estimation of protein content. The methods based on dye binding might provide additional information and substitute the Lowry method if an adequate protein is selected for a calibration plot.

摘要

本文综述了用于估算溶液中蛋白质含量的方法。这些方法基于测量一些与蛋白质结合的有机染料——氨基黑10B、考马斯亮蓝R - 250和G - 250、滂索G、硫酸紫、溴酚蓝、萘酚黄S、橙黄G。将这些方法的优缺点与洛瑞等人(1951年)的方法以及其他一些估算蛋白质含量的传统方法进行了比较。如果为校准曲线选择了合适的蛋白质,基于染料结合的方法可能会提供额外信息并替代洛瑞方法。

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