Kan K S, Chao L P
Muscle Nerve. 1981 Mar-Apr;4(2):91-3. doi: 10.1002/mus.880040203.
Choline acetyltransferase (ChAc) was localized at the neuromuscular junctions of rabbit diaphragm with immunohistochemical staining, which produced a brown color, in combination with histochemical staining for cholinesterase, which produced a blue color, in the same section. The innervations of nerve terminals at the neuromuscular junctions were comparable when a silver impregnation was substituted for the immunohistochemical staining of ChAc.
通过免疫组织化学染色,在兔膈肌的神经肌肉接头处定位了胆碱乙酰转移酶(ChAc),染色后呈棕色,同时在同一切片中进行胆碱酯酶的组织化学染色,染色后呈蓝色。当用银浸染替代ChAc的免疫组织化学染色时,神经肌肉接头处神经末梢的神经支配情况相当。