Noller H F, Woese C R
Science. 1981 Apr 24;212(4493):403-11. doi: 10.1126/science.6163215.
A secondary structure model for 16S ribosomal RNA which is based on available chemical, enzymatic, and comparative sequence data shows good agreement between constraints dictated by the model and a wide variety of experimental observations. The four major structural domains created by the base-pairing scheme correspond closely to RNA fragments isolated after nuclease digestion in the presence of bound ribosomal proteins. Functionally important sites appear to be located in unpaired regions and are phylogenetically highly conserved.
基于现有化学、酶学及比较序列数据构建的16S核糖体RNA二级结构模型显示，该模型所规定的限制条件与大量实验观察结果高度吻合。碱基配对方案形成的四个主要结构域与在结合核糖体蛋白存在下经核酸酶消化后分离出的RNA片段密切对应。功能重要位点似乎位于未配对区域，并且在系统发育上高度保守。
