Kuznetsova V S, Orlova T I, Silaev A B
Antibiotiki. 1981 Jan;26(1):5-7.
A new actinomycin was isolated from a mixture of actinomycins formed by Actinomyces sp. No. 2, an organism producing auranthin, an actinomycetous antibiotic. The peptide chains of the new actinomycin contain such amino acids as threonine, valine, proline and sarcosine in a ratio of 2 : 4 : 2 : 2. N-Methyl-valine characteristic of all actinomycins is replaced in position 5 of both pentapeptide chains of the new actinomycin by valine. The new actinomycin is actinomycin D undermethylated in position 5 by valine. When the growing culture of Actinomyces olivobrunneus producing actinomycin D was exposed to sulfadimesine, an inhibitor of biological methylation, production of actinomycin D0 (sarcosine replaced by glycine in one of the pentapeptide chains) markedly increased, which indicated impairment of the glycine residue methylation. Still, no impairment of the valine residue methylation in position 5 of the pentapeptide chains was observed an no actinomycin with N-methyl-valine replaced by valine was formed.
从一株能产生放线菌黄色素（一种放线菌抗生素）的2号放线菌形成的放线菌素混合物中分离出一种新的放线菌素。新放线菌素的肽链含有苏氨酸、缬氨酸、脯氨酸和肌氨酸等氨基酸，其比例为2:4:2:2。所有放线菌素所特有的N-甲基缬氨酸在新放线菌素两条五肽链的第5位被缬氨酸取代。新放线菌素是在第5位被缬氨酸低甲基化的放线菌素D。当产生放线菌素D的橄榄色链霉菌的生长培养物暴露于生物甲基化抑制剂磺胺二甲嘧啶时，放线菌素D0（其中一条五肽链中的肌氨酸被甘氨酸取代）的产量显著增加，这表明甘氨酸残基甲基化受到损害。然而，未观察到五肽链第5位缬氨酸残基甲基化受到损害，也未形成N-甲基缬氨酸被缬氨酸取代的放线菌素。
