[放线菌素C产生菌中蛋白质V生物合成的缬氨酸来源]
[Source of valine for protein V biosynthesis in a producer of actinomycin C].
作者信息
Alekhova T A, Orlova T I, Silaev A B
出版信息
Antibiotiki. 1977 Jun;22(6):489-92.
The specific activity of 14C-valine in valyl-tRNA formed during incubation of the actinomycin C-producing organism with 14C-valine was constant and lower than that of the whole cell pool. The constancy of the valyl-tRNA was indicative of the presence of a separate compartment for the valine pool used for protein biosynthesis. A lower specific activity of valine in valyl-tRNA as compared to that of the whole cell pool may be indicative of a low rate of valine metabolism in such separate compartment with exogenic 14-valine or a higher concentration of free valine in it as compared to the specific activity of this amino acid at average per cell.
在用¹⁴C-缬氨酸培养放线菌素C产生菌的过程中，所形成的缬氨酰-tRNA中¹⁴C-缬氨酸的比活性恒定，且低于全细胞池的比活性。缬氨酰-tRNA的这种恒定性表明存在一个用于蛋白质生物合成的缬氨酸池的独立区室。与全细胞池相比，缬氨酰-tRNA中缬氨酸的比活性较低，这可能表明在这个独立区室中，外源性¹⁴C-缬氨酸的缬氨酸代谢速率较低，或者其中游离缬氨酸的浓度高于该氨基酸在每个细胞中的平均比活性。
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